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RESEARCH PRODUCT
Protein delivery by subviral particles of human cytomegalovirus
Sandra Pepperl-klindworthNadine FrankenbergBodo PlachterS Rieglersubject
Human cytomegalovirusRecombinant Fusion ProteinsGenetic enhancementGenetic VectorsGreen Fluorescent ProteinsCongenital cytomegalovirus infectionCytomegalovirusGene ExpressionBiologylaw.inventionGreen fluorescent proteinlawVaccines DNAGeneticsmedicineHumansMolecular BiologyKanamycin KinaseSecretory VesiclesLipid bilayer fusionDendritic CellsGenetic TherapyFibroblastsmedicine.diseaseFusion proteinVirologyCell biologyLuminescent ProteinsFluorescent Antibody Technique DirectRecombinant DNAMolecular MedicineDelivery systemGenetic Engineeringdescription
Direct protein delivery is an emerging technology in vaccine development and gene therapy. We could previously show that subviral dense bodies (DB) of human cytomegalovirus (HCMV), a beta-herpesvirus, transport viral proteins into target cells by membrane fusion. Thus these non-infectious particles provide a candidate delivery system for the prophylactic and therapeutic application of proteins. Here we provide proof of principle that DB can be modified genetically. A 55 kDa fusion protein consisting of the green fluorescent protein and the neomycin phosphotransferase could be packed in and delivered into cells by recombinant DB in a functional fashion. Furthermore, transfer of protein into fibroblasts and dendritic cells by DB was efficient, leading to exogenous loading of the MHC-class I antigen presentation pathway. Thus, DB may be a promising basis for the development of novel vaccine strategies and therapeutics based on recombinant polypeptides.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-01-23 | Gene Therapy |