Effect of oxidative stress on UDP-glucuronosyltransferases in rat astrocytes.

6533b870fe1ef96bd12d0694

RESEARCH PRODUCT

Effect of oxidative stress on UDP-glucuronosyltransferases in rat astrocytes.

Alain Minn Daniela Gradinaru Jean-marie Heydel Anne-laure Minn Yves Artur

subject

MESH : Oxidative Stress MESH : RNA Messenger Antioxidant Transcription Genetic medicine.medical_treatment Toxicology NAD(P)H:quinone oxidoreductase 1 MESH: Glucuronosyltransferase Antioxidants Substrate Specificity Rats Sprague-Dawley 0302 clinical medicine MESH: NADPH-Ferrihemoprotein Reductase MESH: Glucuronides NAD(P)H Dehydrogenase (Quinone)MESH : Catalysis MESH: Animals MESH : NAD(P)H Dehydrogenase (Quinone)Glucuronosyltransferase Cells Cultured chemistry.chemical_classification MESH : Cell Survival 0303 health sciences MESH : Substrate Specificity MESH : Animals Newborn Cytochrome P450 reductase General Medicine MESH: Cell Survival MESH: Pyridinium Compounds MESH : Antioxidants MESH: Cells Cultured Oxidative phosphorylation Gene Expression Regulation Enzymologic MESH : Quinones MESH : Glucuronides 03 medical and health sciences RNA Messenger Cell Shape NADPH-Ferrihemoprotein Reductase MESH : Oxidation-Reduction MESH : Pyridinium Compounds MESH: Naphthols MESH : Glucuronosyltransferase MESH: Antioxidants MESH: Catalysis chemistry Oxidative stress Astrocytes Reactive Oxygen Species 030217 neurology & neurosurgery MESH: Oxidation-Reduction Time Factors [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition MESH : Reactive Oxygen Species NADPH:cytochrome P450 reductase Pyridinium Compounds Naphthols MESH: Rats Sprague-Dawley Protein oxidation medicine.disease_cause MESH: Animals Newborn MESH: NAD(P)H Dehydrogenase (Quinone)Protein Carbonylation MESH : Oxidants MESH: Oxidants Melatonin MESH: Melatonin MESH: Oxidative Stress MESH : Melatonin MESH : Rats MESH: Gene Expression Regulation Enzymologic Quinones MESH: Reactive Oxygen Species Oxidants Biochemistry MESH : Protein Carbonylation Oxidation-Reduction UDP-glucuronosyltransferase MESH : Time Factors MESH: Protein Carbonylation MESH: Rats Cell Survival MESH : Naphthols Biology Catalysis MESH: Quinones MESH : Gene Expression Regulation Enzymologic Glucuronides MESH : Cells Cultured medicine Animals MESH: Cell Shape 030304 developmental biology MESH: RNA Messenger Reactive oxygen species MESH: Transcription Genetic MESH: Time Factors MESH : Astrocytes MESH : Transcription Genetic NAD(P)H Dehydrogenase (Quinone)MESH : Rats Sprague-Dawley Rats MESH: Astrocytes Animals Newborn MESH : NADPH-Ferrihemoprotein Reductase MESH: Substrate Specificity MESH : Animals NAD+ kinase MESH : Cell Shape [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Oxidative stress

description

WOS:000309170300003; International audience; The present work reports data regarding effects of an induced oxidative stress on the mainly expressed isoforms of UDP-glucuronosyltransferases (UGTs) in the brain. UGT1A6 and UGT1A7 expression and enzymatic activities toward the 1-naphthol were analyzed in rat cultured astrocytes following the exposure for 48 h to redox-cycling xenobiotic compounds such as quinones and bipyridinium ions. The expression of NADPH:cytochrome P450 reductase and NAD(P)H:quinone oxidoreductase 1 (NQO1) was also investigated. Oxidative stress induced significant deleterious changes in astrocyte morphology, decreased cell viability and inhibited catalytic function of UGTs as a result of protein oxidation. Alternatively, in the surviving impaired astrocytes, oxidative conditions induced a significant overactivity and overexpression of xenobiotic detoxification enzymes, as adaptive response. These effects were significantly prevented by the presence of melatonin, suggesting its direct antioxidant action on reactive oxygen species, reflected further on the glucuronidation activity and transcriptional regulation of both UGT1A6 and UGT1A7. Results show that both catalytic properties of UGTs and the expression of UGT1A6, UGT1A7, NQO1 and NADPH:cytochrome P450 reductase in rat astrocytes are greatly influenced by the pro-oxidative environment. In conclusion, an experimental increase in oxidative cellular status could have both immediate and long term consequences on detoxification enzymatic system activity and expression.

year	journal	country	edition	language
2012-09-18

10.1016/j.toxlet.2012.07.014 https://hal.archives-ouvertes.fr/hal-00767137