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RESEARCH PRODUCT
The effect of water on protein dynamics
G. ZaccaiR. DanielR. HooperF. FranksAntonio Cupanesubject
Dynamical transitionWater activityNeutron diffractionHalophilic malate dehydrogenaseBacteriorhodopsinHydration shellNeutronEuryarchaeotaGeneral Biochemistry Genetics and Molecular BiologyMalate DehydrogenaseSpectroscopySpectrum AnalysibiologyChemistrySpectrum AnalysisProtein dynamicsWaterBacteriorhodopsinPurple membraneAgricultural and Biological Sciences (miscellaneous)Settore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)Neutron DiffractionMembraneSolvation shellAgricultural and Biological Sciences (all)BiochemistryChemical physicsBacteriorhodopsinsbiology.proteinBacteriorhodopsinsGeneral Agricultural and Biological SciencesResearch Articledescription
Neutron diffraction and spectroscopy were applied to describe the hydration and dynamics of a soluble protein and a natural membrane from extreme halophilic Archaea. The quantitative dependence of protein motions on water activity was clearly illustrated, and it was established that a minimum hydration shell is required for the systems to access their functional resilience, i.e. a dynamics state that allows biological activity.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-08-29 | Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences |