6533b871fe1ef96bd12d0f21

RESEARCH PRODUCT

Inhibition of molybdenum blue formation by ATP.

U. Walter

subject

PharmacologyMolybdenumTime zeroChemistryInorganic chemistryCell BiologyPhosphatePhosphatesCellular and Molecular NeuroscienceHydrolysischemistry.chemical_compoundKineticsInorganic phosphateAdenosine TriphosphateMolybdenum blueFixed timeMolecular MedicineIndicators and ReagentsMolecular Biology

description

Molybdenum blue formation was not affected by the presence of ATP up to a concentration of 1.2 muM/l. At higher concentrations the color development was inhibited relative to ATP concentration, finally reaching complete inhibition. Auto-hydrolysis of ATP was found at a rate of 1.4%/h. An exact determination of inorganic phosphate in the presence of easily hydrolyzed phosphate esters requires the measurement of extinction at fixed time intervals and extrapolation back to time zero.

10.1007/bf01948349https://pubmed.ncbi.nlm.nih.gov/7327226