6533b871fe1ef96bd12d0fcb
RESEARCH PRODUCT
High-performance micellar liquid chromatography determination of sulphonamides in pharmaceuticals after azodye precolumn derivatization
M.c. García-alvarez-coqueErnesto F. Simó-alfonsoJosep Esteve-romeroGuillermo Ramis-ramossubject
Quality ControlSulfonamidesChromatographyChemistrySodiumClinical BiochemistryPharmaceutical Sciencechemistry.chemical_elementHydrogen-Ion ConcentrationHigh-performance liquid chromatographyDosage formAnalytical Chemistrychemistry.chemical_compoundColumn chromatographyMicellar liquid chromatographyDrug DiscoveryIndicators and ReagentsSpectrophotometry UltravioletDerivatizationAzo CompoundsQuantitative analysis (chemistry)Chromatography High Pressure LiquidMicellesSpectroscopyAntibacterial agentdescription
Abstract A chromatographic procedure with precolumn derivatization to form the N-(1-naphthyl)ethylenediamine dihydrochloride azodyes is proposed for the analysis of several sulphonamides (sodium sulphacetamide, sulphadiazine, sulphaguanidine, sulphamerazine, sulphamethizole, sulphamethoxazole, sulphanilamide and sulphathiazole) in pharmaceutical preparations (tablets, pills, capsules, suspensions and drops). The separation is performed with a 0.05 M sodium dodecyl sulphate/2.4% pentanol eluent at pH 7. The precolumn derivatization improved the resolution in the chromatograms and increased the selectivity in the determination of mixtures of sulphonamides and in preparations where other drugs were present. The derivatization reaction was readily performed in a micellar medium of SDS at pH 1, leading to a rapid and simple procedure. The recoveries were in the 97–104% range with relative standard deviations usually below 3%.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-03-01 | Journal of Pharmaceutical and Biomedical Analysis |