6533b871fe1ef96bd12d1138

RESEARCH PRODUCT

Antibodies to cell surface ganglioside GD3 perturb inductive epithelial-mesenchymal interactions

Sigrid Henke-fahleWolfgang DippoldPeter EkblomEnno AufderheideHelga BernhardHannu Sariola

subject

medicine.drug_classMesenchymeMorphogenesisFluorescent Antibody TechniqueBiologyKidneyMonoclonal antibodyEpitheliumGeneral Biochemistry Genetics and Molecular BiologyMesodermMiceOrgan Culture TechniquesCell–cell interactionGangliosidesMorphogenesismedicineAnimalsGanglioside GD3Embryonic InductionMembrane GlycoproteinsAntibodies MonoclonalEmbryonic stem cellEpitheliumFibronectinsCell biologyFibronectinmedicine.anatomical_structureBiochemistryAntigens Surfacebiology.proteinUreterCell Adhesion Molecules

description

Abstract Most epithelial sheets emerge during embryogenesis by a branching and growth of the epithelium. The surrounding mesenchyme is crucial for this process. We report that branching morphogenesis and the formation of a new epithelium from the mesenchyme in the embryonic kidney can be blocked by a monoclonal antibody reacting with a surface glycolipid, disialoganglioside G D3 . In contrast, a more than 10-fold excess of antibodies to adhesive glycoproteins (N-CAM, L -CAM, fibronectin) fails to inhibit morphogenesis. Although the anti-G D3 antibody affected epithelial development, the disialoganglioside G D3 was expressed not in the epithelium, but in the mesenchyme surrounding the developing epithelia. The data raise the intriguing possibility that the anti-G D3 antibody inhibits epithelial development by interfering with epithelial-mesenchymal interactions.

yearjournalcountryeditionlanguage
1988-07-01Cell
https://doi.org/10.1016/0092-8674(88)90556-9