A rapid method for the differentiation of yeast cells grown under carbon and nitrogen-limited conditions by means of partial least squares discriminant analysis employing infrared micro-spectroscopic data of entire yeast cells.

6533b871fe1ef96bd12d1148

RESEARCH PRODUCT

A rapid method for the differentiation of yeast cells grown under carbon and nitrogen-limited conditions by means of partial least squares discriminant analysis employing infrared micro-spectroscopic data of entire yeast cells.

Christoph Herwig Guillermo Quintás Bernhard Lendl Julia Kuligowski

subject

Time Factors Chemistry(all)Spectrophotometry Infrared Nitrogen Analytical chemistry Infrared spectroscopy Pichia Article Analytical Chemistry Pichia pastoris Pichia pastoris Infrared (IR) micro-spectroscopy Partial least squares regression Process control Partial least squares-discriminant analysis (PLS-DA)Least-Squares Analysis Projection (set theory)Cell Proliferation Principal Component Analysis biology Chemistry Discriminant Analysis Reproducibility of Results Linear discriminant analysis biology.organism_classification Double cross validation (2CV)Yeast Carbon Yeast Culture Media Permutation testing Principal component analysis Feasibility Studies Biological system

description

This paper shows the ease of application and usefulness of mid-IR measurements for the investigation of orthogonal cell states on the example of the analysis of Pichia pastoris cells. A rapid method for the discrimination of entire yeast cells grown under carbon and nitrogen-limited conditions based on the direct acquisition of mid-IR spectra and partial least squares discriminant analysis (PLS-DA) is described. The obtained PLS-DA model was extensively validated employing two different validation strategies: (i) statistical validation employing a method based on permutation testing and (ii) external validation splitting the available data into two independent sub-sets. The Variable Importance in Projection scores of the PLS-DA model provided deeper insight into the differences between the two investigated states. Hence, we demonstrate the feasibility of a method which uses IR spectra from intact cells that may be employed in a second step as an in-line tool in process development and process control along Quality by Design principles.

year	journal	country	edition	language
2012-09-01	Talanta

10.1016/j.talanta.2012.06.036 https://pubmed.ncbi.nlm.nih.gov/22967595