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RESEARCH PRODUCT
Evidence for a novel cytoplasmic processing event in ribosome maturation in the sea urchin Paracentrotus lividus
Daniele BellaviaRainer Barbierisubject
In situCytoplasmSea urchinEmbryo NonmammalianRibosome maturation ; Processing ; Shuttling ; Sea urchin ; Pre-rRNAsSea Urchin ribosome maturation rRNA.ProcessingRibosomePrimer extensionParacentrotus lividusCellular and Molecular Neurosciencebiology.animalRNA PrecursorsmedicineAnimalsRNA Processing Post-TranscriptionalMolecular BiologySea urchinIn Situ HybridizationPharmacologybiologyPre-rRNAsCell BiologyRibosomal RNAbiology.organism_classificationMolecular biologyCell biologySettore BIO/18 - Geneticamedicine.anatomical_structureShuttlingCytoplasmOocytesParacentrotusMolecular MedicineFemaleRibosomesRibosome maturationNucleusdescription
In this work, we demonstrate the existence of a cytoplasmic processing step, never before described, involving both the pre-ribosomal subunits in the sea urchin Paracentrotus lividus. Northern-blot hybridization, primer extension, S1 mapping experiments and in situ hybridizations allowed us to demonstrate that cytoplasmic processed particles are successively re-imported into the nucleus, where maturation of their RNAs is completed prior to being exported to the cytoplasm. Our findings lead to the proposal of a new model of ribosome maturation and shuttling. Moreover, preliminary data from our laboratory suggest that the maturation pathway we propose in P. lividus may not be unique to the sea urchin, but a common feature in eukaryotes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-01-01 | Cellular and Molecular Life Sciences |