6533b871fe1ef96bd12d19ab
RESEARCH PRODUCT
Design and development of hyaluronan-functionalized polybenzofulvene nanoparticles as CD44 receptor mediated drug delivery system
Gaetano GiammonaMarco PaolinoAndrea CappelliFrancesco MakovecCinzia ScialabbaVincenzo RazzanoGiorgio GrisciMariano LicciardiGermano Giulianisubject
DrugMaterials scienceAtomic and Molecular Physics and Opticmedia_common.quotation_subjectHyaluronic acidCD44 receptorBioengineering02 engineering and technologyPharmacology010402 general chemistry01 natural sciencesCD44 receptor; Doxorubicin; Hyaluronic acid; Nanomedicine; Polybenzofulvene; Tri-component polymer brush TCPB; Bioengineering; Chemistry (all); Atomic and Molecular Physics and Optics; Modeling and Simulation; Materials Science (all); Condensed Matter Physicschemistry.chemical_compoundAtomic and Molecular PhysicsHyaluronic acidTri-component polymer brush TCPBmedicineGeneral Materials ScienceDoxorubicinmedia_commonbiologyCD44Chemistry (all)General Chemistry021001 nanoscience & nanotechnologyCondensed Matter PhysicsAtomic and Molecular Physics and Optics0104 chemical sciencesNanomedicinechemistryTargeted drug deliveryDoxorubicinModeling and SimulationDrug deliveryCancer cellbiology.proteinBiophysicsNanomedicineMaterials Science (all)Polybenzofulveneand Optics0210 nano-technologymedicine.drugdescription
A tri-component polymer brush (TCPB), composed of a polybenzofulvene copolymer bearing low molecular weight hyaluronic acid (HA) on the surface of its cylindrical brush-like backbone and oligo-PEG fractions, was employed in the preparation of 350Â nm nanostructured drug delivery systems capable of delivering the anticancer drug doxorubicin. The obtained drug delivery systems were characterized on the basis of drug loading and release, dimensions and zeta potential, morphology and in vitro cell activity, and uptake on three different human cell lines, namely the bronchial epithelial 16HBE, the breast adenocarcinoma MCF-7, and the colon cancer HCT116 cells. Finally, the ability of doxorubicin-loaded TCPB nanoparticles (DOXO-TCPB) to be internalized into cancer cells by CD44 receptor mediated uptake was assessed by means of uptake studies in HCT cells. These data were supported by anti-CD44-FITC staining assay. The proposed TCPB nanostructured drug delivery systems have many potential applications in nanomedicine, including cancer targeted drug delivery.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-06-01 |