6533b871fe1ef96bd12d19b5
RESEARCH PRODUCT
Isolation and purification of plasmids from Bacteroides fragilis using rubidium trichloroacetate density gradient centrifugation.
E. Jürgen ZöllnerFranz-peter SchwindlingChristoph Von Eichel-streibersubject
Plasmid preparationDifferential centrifugationDNA BacterialRNAchemistry.chemical_elementBiologyIsolation (microbiology)RubidiumMolecular biologyRubidiumBacteroides fragilisPlasmidchemistryGeneticsCentrifugation Density GradientUltracentrifugePurification methodsTrichloroacetic AcidMolecular BiologyPlasmidsdescription
A rapid and easy final purification method is described for the isolation of plasmids from B. fragilis. Using RbTCA density gradient centrifugation in an airfuge ultracentrifuge ccc plasmid DNA can be separated from RNA, residual chromosomal DNA, linear and oc plasmid DNA. Pure ccc plasmid DNA is obtained from cultures of between 1 ml and 2 l in less than one day.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1983-02-01 | Moleculargeneral genetics : MGG |