6533b871fe1ef96bd12d19da
RESEARCH PRODUCT
Changes in gene expression during adaptation of Listeria monocytogenes to the soil environment
Géraldine DepretGéraldine DepretPascal PiveteauPascal PiveteauDominique GarmynDominique GarmynBarbara PivatoBarbara PivatoAlain HartmannAlain Hartmannsubject
listeriaTime Factorslisteria monocytogenes[SDV]Life Sciences [q-bio]Gene ExpressionATP-binding cassette transporterSoil Chemistrymedicine.disease_causemicroorganisme du solPhosphotransferaseTranscriptomeSoilMolecular Cell BiologySoil MicrobiologyOligonucleotide Array Sequence Analysisbactérie2. Zero hungerRegulation of gene expression0303 health sciencesMultidisciplinaryReverse Transcriptase Polymerase Chain ReactionQRGene Expression Regulation DevelopmentalAdaptation PhysiologicalBacterial PathogensChemistry[SDE]Environmental SciencesMedicinelisteria monocytogenes ;relation sol microorganismeSoil microbiologyResearch ArticleScienceEnvironmentBiologyMicrobiologyMicrobial EcologyMicrobiology03 medical and health sciencesbiologie du solListeria monocytogenesmedicineEnvironmental ChemistrylistérioseBiologyEcosystem030304 developmental biologyrelation sol microorganismeGram Positiveécologie microbienne030306 microbiologyCatabolismGene Expression ProfilingGene Expression Regulation BacterialRegulonTranscriptomedescription
project SEST 009; International audience; Listeria monocytogenes is a ubiquitous opportunistic pathogen responsible for listeriosis. In order to study the processes underlying its ability to adapt to the soil environment, whole-genome arrays were used to analyse transcriptome modifications 15 minutes, 30 minutes and 18 h after inoculation of L. monocytogenes EGD-e in soil extracts. Growth was observed within the first day of incubation and large numbers were still detected in soil extract and soil microcosms one year after the start of the experiment. Major transcriptional reprofiling was observed. Nutrient acquisition mechanisms (phosphoenolpyruvate-dependent phosphotransferase systems and ABC transporters) and enzymes involved in catabolism of specific carbohydrates (b-glucosidases; chitinases) were prevalent. This is consistent with the overrepresentation of the CodY regulon that suggests that in a nutrient depleted environment, L. monocytogenes recruits its extensive repertoire of transporters to acquire a range of substrates for energy production.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-01-01 |