6533b871fe1ef96bd12d19e3
RESEARCH PRODUCT
Induction of Apoptosis, Autophagy and Ferroptosis by Thymus vulgaris and Arctium lappa Extract in Leukemia and Multiple Myeloma Cell Lines
Alaadin M. NaqishbandiMohamed-elamir F. HegazyThomas A. EfferthAveen Nozad AdhamAveen Nozad Adhamsubject
Programmed cell deathautophagyThymus vulgarisPharmaceutical ScienceNobiletinAnalytical Chemistrylcsh:QD241-441lamiaceae03 medical and health scienceschemistry.chemical_compound0302 clinical medicineUrsolic acidlcsh:Organic chemistryDrug DiscoveryasteraceaePhysical and Theoretical ChemistryCytotoxicity030304 developmental biology0303 health sciencesbiologyOrganic Chemistryapoptosisphytotherapybiology.organism_classificationMolecular biologyferroptosismultiple myelomacell deathchemistryChemistry (miscellaneous)Apoptosis030220 oncology & carcinogenesisArctium lappaApigeninMolecular Medicinedescription
Thymus vulgaris and Arctium lappa have been used as a folk remedy in the Iraqi Kurdistan region to deal with different health problems. The aim of the current study is to investigate the cytotoxicity of T. vulgaris and A. lappa in leukemia and multiple myeloma (MM) cell lines and determine the mode of cell death triggered by the most potent cytotoxic fractions of both plants in MM. Resazurin assay was used to evaluate cytotoxic and ferroptosis activity, apoptosis, and modulation in the cell cycle phase were investigated via Annexin V-FITC/PI dual stain and cell-cycle arrest assays. Furthermore, we used western blotting assay for the determination of autophagy cell death. n-Hexane, chloroform, ethyl acetate, and butanol fractions of T. vulgaris and A. lappa exhibited cytotoxicity in CCRF-CEM and CEM/ADR 5000 cell lines at concentration range 0.001&ndash
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-10-29 | Molecules |