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RESEARCH PRODUCT

Miniaturized matrix solid phase dispersion procedure and solid phase microextraction for the analysis of organochlorinated pesticides and polybrominated diphenylethers in biota samples by gas chromatography electron capture detection

Yolanda Moliner-martínezPilar Campíns-falcóA. Seco-torrecillasCarmen Molins-leguaL. Segovia-martínez

subject

Detection limitChromatography GasChromatographyChemistryOrganic ChemistryPesticide ResiduesAnalytical chemistryGeneral MedicineSolid-phase microextractionBiochemistryDispersantBivalviaAnalytical ChemistrySolventElectron capture detectorHalogenated Diphenyl EthersHydrocarbons ChlorinatedAnimalsSample preparationSolid phase extractionGas chromatographyFood AnalysisSolid Phase Microextraction

description

Abstract This work has developed a miniaturized method based on matrix solid phase dispersion (MSPD) using C18 as dispersant and acetonitrile–water as eluting solvent for the analysis of legislated organochlorinated pesticides (OCPs) and polybrominated diphenylethers (PBDEs) in biota samples by GC with electron capture (GC-ECD). The method has compared Florisil®-acidic Silica and C18 as dispersant for samples as well as different solvents. Recovery studies showed that the combination of C18–Florisil® was better when using low amount of samples (0.1 g) and with low volumes of acetonitrile–water (2.6 mL). The use of SPME for extracting the analytes from the solvent mixture before the injection resulted in detection limits between 0.3 and 7.0 μg kg−1 (expressed as wet mass). The miniaturized procedure was easier, faster, less time consuming than the conventional procedure and reduces the amounts of sample, dispersant and solvent volume by approximately 10 times. The proposed procedure was applied to analyse several biota samples from different parts of the Comunidad Valenciana.

yearjournalcountryeditionlanguage
2009-06-11Journal of Chromatography A
https://doi.org/10.1016/j.chroma.2009.08.021