6533b871fe1ef96bd12d1b65
RESEARCH PRODUCT
Direct Identification of Each Specific Mutation in Codon 12 and 13 of ci-ki-ras2 by SSCP Analysis
Mario La FarinaNatascia MaturiIda AlbaneseSalvatore StiraViviana BazanAntonio Russosubject
SSCP analysisBiophysicsBiologyBiochemistryFrameshift mutationProto-Oncogene Proteins p21(ras)chemistry.chemical_compoundGene FrequencyHumansCloning MolecularRas2CodonMolecular BiologyPolymorphism Single-Stranded ConformationalSequence (medicine)GeneticsSpecific mutationCarcinomaWild typeSingle-strand conformation polymorphismDNA NeoplasmCell BiologyMolecular biologyGenes raschemistryMutationElectrophoresis Polyacrylamide GelColorectal NeoplasmsDNAdescription
We compared the SSCP behaviour of the DNA fragments containing c-ki-ras 2 wild type 12 and 13 codons or each of the 12 possible point mutated sequences in these two codons. We found that a single electrophoresis condition was sufficient to distinguish each specific mutation from the other 11 and from the wild type sequence. This observation makes it possible to identify each specific mutation directly by SSCP without any need for reamplification and sequencing.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-05-01 | Biochemical and Biophysical Research Communications |