6533b871fe1ef96bd12d1c19

RESEARCH PRODUCT

Microspore Embryogenesis Through Anther Culture in Citrus clementina Hort. ex Tan.

Maria GermanaBenedetta Chiancone

subject

0106 biological sciences0301 basic medicineClementineAnther cultureCitruStamenMicrospore embryogenesiPlant DevelopmentGerminationBreedingHaploidyBiologymedicine.disease_cause01 natural sciencesGenetic analysis03 medical and health sciencesMicrosporePollenBotanyGeneticsmedicinePlant breedingCultivarMolecular BiologyPlant Somatic Embryogenesis TechniqueSomatic embryogenesifungiEmbryogenesisGametic embryogenesifood and beveragesSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeTransformation (genetics)030104 developmental biologyIsolated microspore cultureDoubled haploidPollenTissue Culture TechniqueGenome Plant010606 plant biology & botany

description

Anther culture is a biotechnological method that allows to obtain, in one step, homozygous plants, very important to plant breeding, due to their numerous applications in mutation research, selection, genome sequencing, genetic analysis, and transformation. To induce the microspores, i.e., the immature male gametes, to switch from the normal gametophytic pathway to the sporophytic one, it is necessary to submit them to a type of stress, such as high or low temperature, starvation, or magnetic field. Stress can be applied to the donor plants and/or the fl oral buds or the anthers or the isolated microspores, before or during the culture. In this chapter, the protocol to induce gametic embryogenesis from anther culture of several cultivars of Citrus clementina Hort. ex Tan. is reported.

yearjournalcountryeditionlanguage
2016-01-01
https://doi.org/10.1007/978-1-4939-3061-6_27