Trīs jaunu Siphoviridae dzimtai piederošu bakteriofāgu genoma un proteoma raksturošana

6533b871fe1ef96bd12d1e9b

RESEARCH PRODUCT

Trīs jaunu Siphoviridae dzimtai piederošu bakteriofāgu genoma un proteoma raksturošana

ŅIkita Zrelovs

subject

in silico genoma anotēšana bakteriofāgi genoma sekvenēšana Bioloģija proteīnu homoloģija

description

Darbā pētīti trīs Siphoviridae bakteriofāgi no BMC DNS-fāgu kolekcijas. Papildus molekulārās bioloģijas metodēm darbā plaši lietotas in silico metodes. Iegūtās fāgu genomu consensus sekvences tika validētas ar atbilstošiem eksperimentāliem datiem. Fāgu genomi tika anotēti un tie uzrādīja augstu oriģinalitātes pakāpi. Tika konstatēts, ka jauniegūto fāgu proteomos atrodami daudzi evolucionāri konservatīvi ORF produkti, kuru homologi sastopami dažādos organismos, bet kuru funkcija paliek nezināma, līdz ar to tie kalpo par kandidātiem turpmākiem proteīnu strukturāli-funkcionāliem pētījumiem. in silico modulēšana ļāva izvēlēties turpmākās in vitro darbības genomu fizisko galu un DNS pakošanas stratēģijas noteikšanai. Pētīto fāgu genomu sekvences šobrīd tiek gatavotas deponēšanai gēnu bankā.

year	journal	country	edition	language
2018-01-01

https://dspace.lu.lv/dspace/handle/7/38618

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