6533b871fe1ef96bd12d2502
RESEARCH PRODUCT
T lymphocyte control of autoreactivity: analysis with human T cell clones and limiting dilution culture
Stefan MeuerUlrich MoebiusKarl-hermann Meyer Zum BüschenfeldeKai Deuschsubject
Cytotoxicity Immunologiceducation.field_of_studyT-LymphocytesT cellImmunologyPopulationHistocompatibility Antigens Class IICell CommunicationT lymphocyteBiologyT-Lymphocytes RegulatoryPeripheral blood mononuclear cellClone CellsCell biologymedicine.anatomical_structureCell–cell interactionCell cultureImmunologyImmune TolerancemedicineHumansImmunology and AllergyCytotoxic T cellClone (B-cell biology)educationdescription
To investigate cellular mechanisms controlling activated autoreactive T lymphocytes, a limiting dilution system was established employing cloned autoreactive major his-tocompatibility complex class II specific lymphocytes (a2/7) as stimulator cells for autologous peripheral blood mononuclear cells. At low responder/stimulator ratios, cytotoxic effector cells were generated capable of lysing clone a2/7. Importantly, within the population of cells mediating autocytotoxic effector function, differential specificities were found to exist. The generation of such autocytotoxic T lymphocytes appears to be inhibited by an additional population of cells circulating at lower frequency suggesting that autoreactivity is controlled at distinct levels by discrete functional cellular subsets.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1986-11-01 | European Journal of Immunology |