Modulation of lipid metabolism and colonic microbial diversity of high-fat-diet C57BL/6 mice by inulin with different chain lengths

6533b871fe1ef96bd12d260b

RESEARCH PRODUCT

Modulation of lipid metabolism and colonic microbial diversity of high-fat-diet C57BL/6 mice by inulin with different chain lengths

Qian Wu Xiao Luo Jingren He Yuqi Huang Shuyi Li Francisco J. Barba Zhenzhou Zhu

subject

Glycation End Products Advanced Male Colon 030309 nutrition & dietetics Inulin Gut flora Diet High-Fat Antioxidants Catechin Mice 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Litchi Glycation Malondialdehyde RNA Ribosomal 16S Animals Biflavonoids Ingestion Proanthocyanidins Food science Liver X Receptors chemistry.chemical_classification Glutathione Peroxidase 0303 health sciences Sterol response element binding biology Glutathione peroxidase Body Weight Cholesterol HDL Inulin Lipid metabolism Cholesterol LDL 04 agricultural and veterinary sciences Lipid Metabolism Malondialdehyde biology.organism_classification 040401 food science Gastrointestinal Microbiome Mice Inbred C57BL Liver chemistry Acyl Coenzyme A Sterol Regulatory Element Binding Protein 1 ATP Binding Cassette Transporter 1 Food Science

description

Abstract The physicochemical properties, biological functions and microbial degradation of inulins differ according to their degree of polymerization. However, the relationship between inulin activities and its effect on gut microbiota remains unknown. In this study, high fat diet with inulin (1 or 5 g/kg·bw), either with short or long chains groups were administered to different groups of mice (n = 10) for 10 weeks in order to investigate the effect of inulin on the microbial diversity of the animals. Litchi pericarp procyanidins (LPPC) were used for comparison purposes. Furthermore, the lipid metabolism and key regulator genes in mice were determined. The results indicated that natural inulin (1 g/kg·bw) ingestion reduced the body weight of fat mice between week 6–9. Glutathione peroxidase (GSH-Px) activity in liver was remarkably higher after adding long chain inulin (5 g/kg·bw) compared to high-fat-diet mice. Moreover, high dose of natural inulin regulated malondialdehyde and advanced glycation end-products levels in mice liver. Likewise, the high dose of short-chain inulin increased sterol response element binding protein 1 (SREBP-1), β-Hydroxy β-methylglutaryl-CoA (HMG-CoA) and ATP-binding cassette transporter A1 (ABCA1) genetic expression. A significant change on the abundance of six genera in gut microbial profile suggested that inulin has the ability to modulate the lipid metabolism regardless of chain length, mainly due to its impact on colon microbiota variety.

year	journal	country	edition	language
2019-02-24	Food Research International

https://doi.org/10.1016/j.foodres.2019.05.003