Legionella pneumophila identifikācija, izmantojot MALDI-TOF MS un SBT metodes

6533b871fe1ef96bd12d28b4

RESEARCH PRODUCT

Legionella pneumophila identifikācija, izmantojot MALDI-TOF MS un SBT metodes

Andžela Staškeviča

subject

identifikācija MALDI-TOF MS SBT Bioloģija Legionella pneumophila

description

Legionella pneumophila identifikācijai un subtipēšanai tika pielietotas divas metodes – MALDI-TOF MS un SBT. Tika izmantoti 22 no ūdens izolēti ZI ”BIOR” Legionella pneumophila celmi. Ar lateksa aglutinācijas metodi tika noteiktas izmantojamās Legionella pneumophila serogrupas – 1, 2, 3 un 6. MALDI-TOF MS ekstrakcijas metode nodrošināja 100 % identifikāciju visiem Legionella pneumophila izolātiem. Pēc 17 iegūtajiem masas spektra signāliem celmi tika efektīvi subtipēti. Ar SBT metodi celmiem tika iegūti alēļu profili un sekvences tipa numuri, kopumā 15 dažādi Legionella pneumophila ST, no kuriem divi – ST2191 un ST2192 iepriekš nebija iekļauti starptautiskajā ESGLI datubāzē. Abu metožu iegūto dendrogrammu A klāsterī esošo celmu sakritība sastāda 100 %. Kopējā celmu sakritība abās dendrogrammās – 77, 3 %. MSP dendrogrammas C klāstera celmu sakritība ar SBT dendrogrammas B klāstera B1 daļu ir 80 %, bet B klāstera celmu sakritība ar B2 daļu ir 67 %. Pēc iegūtajiem rezultātiem var secināt, ka MALDI-TOF MS ir efektīva subtipēšanas metode, kuru pilnveidojot, iespējams aizstāt laikietilpīgo un dārgo SBT metodi.

year	journal	country	edition	language
2016-01-01

https://dspace.lu.lv/dspace/handle/7/33177

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