6533b872fe1ef96bd12d2fdd
RESEARCH PRODUCT
A microplate version of the SOS/umu-test for rapid detection of genotoxins and genotoxic potentials of environmental samples
Y. OdaRudolf K. ZahnGeorg ReifferscheidJ. Heilsubject
Salmonella typhimuriumAzidesEnvironmental pollutionToxicologyRiver waterRapid detectionMicrobiologyIndustrial waste waterGeneticsSOS responseSOS Response GeneticsSodium AzideIncubationChromatographyMutagenicity TestsChemistryGenetically engineeredGene Expression Regulation BacterialHydrogen-Ion Concentrationbeta-GalactosidaseDilutionMutagenesisMicrosomes LiverWater Pollutants ChemicalEnvironmental MonitoringMutagensdescription
Abstract The umu-microtest is a miniaturized automated short-term test version proposed for screening of umuC-dependent mutagenic potentials of chemicals relevant to environmental pollution, river water and industrial waste water. The test is based on the SOS/umu-test and has been modified in order to allow extensive testing of environmental samples. Genetically engineered Salmonella typhimurium (TA1535/pSK1002) are incubated on a microplate rotor in a sloping position for 2 h with the test samples, followed by addition of fresh culture medium to reach a 10-fold dilution of the incubation medium. 2 h later, the activity of the β-galactosidase, which reflects umuC induction, is determined colorimetrically. The incubation of the bacteria in the presence of the test compounds as well as the assessment of β-galactosidase activity takes place in 96-well microplates, thus enabling simultaneous screening of large numbers of samples. Data of the genotoxic potentials are available within 8 h. Computer-controlled automation is possible by using a laboratory workstation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-12-01 | Mutation Research/Environmental Mutagenesis and Related Subjects |