6533b872fe1ef96bd12d3480
RESEARCH PRODUCT
Mid-region PTHrP and gene expression of MDA-MB231 breast cancer cells
Sirchia RClaudio Luparellosubject
breast cancerPCRgene expressionPTHrP.differential displaySettore BIO/06 - Anatomia Comparata E Citologiadescription
Type V collagen is known to be over-deposited in the stroma of ductal infiltrating carcinomas of the breast. When used as a substrate, type V collagen restrains growth and invasion, and affects gene expression of 8701-BC ductal infiltrating carcinomas cells. Here we supplement existing data by demonstrating type V collagen dependent upregulation of capn2 gene expression in 8701-BC cells through differential display-PCR and Western blot assays. Furthermore, we suggest that our data obtained by centrifugal sedimentation and electrophoresis strongly suggest a correlation between calpain overproduction and DNA fragmentation, since the incubation with calpain inhibitor partly reverts the latter.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2007-01-01 |