6533b872fe1ef96bd12d38d7
RESEARCH PRODUCT
Performance of interdigitated nanoelectrodes for electrochemical DNA biosensor.
Alain DereuxOlivier SiriEric FinotGuillaume LegayEric BourillotPierre BraunsteinMoustapha Cherkaoui-malkiYvon LacrouteNorbert LatruffeRita Meunier-prestsubject
ChemistryDNA Single-StrandedNucleic Acid HybridizationNanotechnologyBiosensing TechniquesElectrochemistryAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsElectrochemical cellMicroelectrodeElectrodeElectrochemistryAdsorptionGoldInstrumentationLithographyBiosensorVoltammetryMicroelectrodesElectron-beam lithographydescription
An electrochemical methodology for bio-molecule sensing using an array of well-defined nanostructures is presented. We describe the fabrication by e-beam lithography of nanoelectrodes consisting of a 100 micro m x 50 micro m area containing interdigitated electrodes of 100 nm in width and interelectrode distance of 200 nm. Sensitivity and response time of the nanoelectrodes are compared to the responses of macro- and microelectrodes. The specificity of the sensor is studied by modifying the gold electrodes with DNA. The technique enables to characterize both single and double-stranded DNA of 15 nucleotides. A special electrochemical cell is adapted to control the temperature and measure the DNA concentration by UV analysis. The electrochemical method requires no label on the DNA, only redox mediators were used.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-10-01 | Ultramicroscopy |