6533b872fe1ef96bd12d38d9
RESEARCH PRODUCT
Prediction model for aneuploidy in early human embryo development revealed by single-cell analysis.
Maria Vera-rodriguezShawn L. ChavezCarlos SimónRenee A. Reijo PeraCarmen Rubiosubject
animal structuresCellular differentiationGeneral Physics and AstronomyAneuploidyBiologyModels BiologicalGeneral Biochemistry Genetics and Molecular BiologyArticleTranscriptomeSingle-cell analysismedicineHumansGenetic TestingMitosisCell ProliferationGeneticsMultidisciplinaryGene Expression ProfilingGene Expression Regulation DevelopmentalEmbryoCell DifferentiationGeneral Chemistrymedicine.diseaseAneuploidyGene expression profilingembryonic structuresPloidydescription
Aneuploidies are prevalent in the human embryo and impair proper development, leading to cell cycle arrest. Recent advances in imaging and molecular and genetic analyses are postulated as promising strategies to unveil the mechanisms involved in aneuploidy generation. Here we combine time-lapse, complete chromosomal assessment and single-cell RT–qPCR to simultaneously obtain information from all cells that compose a human embryo until the approximately eight-cell stage (n=85). Our data indicate that the chromosomal status of aneuploid embryos (n=26), including those that are mosaic (n=3), correlates with significant differences in the duration of the first mitotic phase when compared with euploid embryos (n=28). Moreover, gene expression profiling suggests that a subset of genes is differentially expressed in aneuploid embryos during the first 30 h of development. Thus, we propose that the chromosomal fate of an embryo is likely determined as early as the pronuclear stage and may be predicted by a 12-gene transcriptomic signature.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-09-26 | Nature communications |