6533b872fe1ef96bd12d395a

RESEARCH PRODUCT

Endometrial stromal sarcomas: immunohistochemical, electron microscopical and cytogenetic findings in two cases.

Carmen CardaAntonio Llombart-boschConcha López-ginésSamuel NavarroRosario Gil-benso

subject

Pathologymedicine.medical_specialtyStromal cellSarcoma Endometrial StromalChromosomes Human Pair 20VimentinChromosome DisordersPathology and Forensic MedicineImmunoenzyme TechniquesFatal OutcomeComplex KaryotypemedicineBiomarkers TumorHumansMolecular BiologyAgedChromosome AberrationsbiologyCytogeneticsKaryotypeHistologyCell BiologyGeneral MedicineGene rearrangementMiddle Agedmedicine.diseaseCombined Modality TherapyChromosome BandingEndometrial NeoplasmsMicroscopy ElectronKaryotypingbiology.proteinChromosomes Human Pair 6FemaleSarcoma

description

Uterine sarcomas are approximately 3% of all malignant uterine corpus tumours. Of these, the tumours that originate solely in the stromal elements of the uterine wall are infrequent and have not been well characterized cytogenetically. We report two cases of endometrial stromal sarcomas (ESS), one low grade and one high grade, diagnosed by conventional histology, immunocytochemistry, electron microscopy and cytogenetics. Morphologically clear-cut differential structures were seen at optical, immunohistochemical, and electron microscopic levels, permitting a clear differential diagnosis. The low-grade ESS expressed hormonal receptors and vimentin, whereas the high-grade ESS showed no hormone receptors, high Ki-67 activity, and occasional cytokeratin-positive cells. Ultrastructurally, no malignant epithelial differentiation was seen in the tumour cells, but cilia were found in both cases. Cytogenetic study of the low-grade ESS showed pseudodiploid karyotype with chromosomes 6 and 20 rearranged. The high-grade ESS showed a complex karyotype with clonal numerical and structural anomalies. The chromosomes involved in the structural rearrangements were 1, 3, 6, 7, 13, 14, 15, 17, 19, and 21.

10.1007/s004280050346https://pubmed.ncbi.nlm.nih.gov/10335941