6533b872fe1ef96bd12d3a9a

RESEARCH PRODUCT

Electron paramagnetic resonance (EPR) spin trapping of biological nitric oxide

Philip WenzelThomas MünzelAndrei L. Kleschyov

subject

ChromatographySpin trappingIronClinical BiochemistryElectron Spin Resonance SpectroscopyCell BiologyGeneral MedicineNitric OxidePhotochemistryBiochemistryAnalytical ChemistryNitric oxidelaw.inventionchemistry.chemical_compoundEpr spin trappingNuclear magnetic resonancechemistryThiocarbamateslawAnimalsHumansElectron paramagnetic resonanceSpin TrappingVolume concentration

description

Nitric oxide (NO) is a free radical species with multiple physiological functions. Because of low concentrations and short half-life of NO, its direct measurement in living tissues remains a difficult task. Electron paramagnetic resonance (EPR) spin trapping is probably one of the best suitable platforms for development of new methods for quantification of biological NO. The most reliable EPR-based approaches developed so far are based on the reaction of NO with various iron complexes, both intrinsic and exogenously applied. This review is focused on the current state and perspectives of EPR spin trapping for experimental and clinical NO biology.

yearjournalcountryeditionlanguage
2007-05-01Journal of Chromatography B
https://doi.org/10.1016/j.jchromb.2006.10.006