6533b872fe1ef96bd12d3aab
RESEARCH PRODUCT
Tobacco cells contain a protein, immunologically related to the neutrophil small G protein Rac2 and involved in elicitor-induced oxidative burst.
B.f. MaumeJean-pierre BleinFlore KiefferFrançoise Simon-plassubject
0106 biological sciencesHypersensitive responseNicotiana tabacumBlotting WesternBiophysicsSmall G Protein01 natural sciencesBiochemistrySuperoxide dismutaseFungal Proteins03 medical and health sciencesStructural BiologyGTP-Binding ProteinsTobaccoGeneticsMolecular BiologyCells Cultured030304 developmental biologyRespiratory Burst0303 health sciencesNADPH oxidasebiologyNADPH oxidaseNicotiana tabacumAlgal Proteinsfood and beveragesCell Biologybiology.organism_classificationMolecular biologyOxidative burst3. Good healthElicitorRespiratory burstrac GTP-Binding ProteinsSmall G proteinCytosolPlants ToxicBiochemistrybiology.proteinCryptogeinReactive Oxygen Species010606 plant biology & botanyRac2description
Abstract Suspension-cultured cells of Nicotiana tabacum generated active oxygen species (AOS) when they were treated with the proteinaceous elicitor, cryptogein. This response was blocked by diphenylene iodonium, an inhibitor of the neutrophil NADPH oxidase. When microsomal extracts of tobacco cells were probed with an antibody directed against the human small G protein Rac2, two immunoreactive proteins were detected at 18.5 and 20.5 kDa. The same experiment performed with cytosolic extracts of tobacco cells led to the observation of a strong immunoreactive protein at 21.5 kDa only in the cryptogein-treated cells. The appearance of this cytosolic protein was related to the production of AOS by the elicited cells. These results provide evidence for the possible involvement of small G proteins, homologous to the neutrophil Rac2 protein, in the regulation of the elicitor-induced oxidative burst in plant. © 1997 Federation of European Biochemical Societies.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-02-17 | FEBS letters |