6533b872fe1ef96bd12d42be
RESEARCH PRODUCT
Characterization of rodent pineal astrocytes by immunofluorescence microscopy using a monoclonal antibody (J1-31).
S. K. MalhotraHannsjörg Schrödersubject
Maleendocrine systemHistologymedicine.drug_classGuinea PigsFluorescent Antibody TechniqueMonoclonal antibodyPineal GlandPathology and Forensic MedicinePineal glandmedicineAnimalsHumansAntiserumCerebral CortexGlial fibrillary acidic proteinbiologyAntibodies MonoclonalRats Inbred StrainsCell BiologyGFAP stainMolecular biologyStainingRatsmedicine.anatomical_structurenervous systemAstrocytesbiology.proteinImmunohistochemistryAntibodyGerbillinaedescription
In previous studies pineal astrocytes have been characterized immunohistochemically mainly by use of antisera to glial fibrillary acidic protein. Because of the recent demonstration of this protein in non-astrocytic cells the question of its specificity as an astrocytic marker has been raised. A possible alternative tool for characterizing pineal astrocytes is the J1-31 monoclonal antibody, which is directed against a 30 000 dalton astrocytic protein clearly distinguishable from glial fibrillary acidic protein. Immunofluorescence microscopy of this antibody in the pineal gland of rat and guinea-pig revealed a staining pattern similar to that obtained by glial acidic fibrillary protein antisera. In the rat, J1-31-immunoreactive cells and processes were concentrated in the transitional region between the superficial pineal gland and pineal stalk. Fibrillar J1-31-immunoreactive structures were seen in the most proximal part of the guinea-pig pineal gland. The J1-31 monoclonal antibody therefore appears to be a useful tool for the demonstration of pineal astrocytes; it avoids the specificity problems of glial fibrillary acidic protein immunohistochemistry.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1987-06-01 | Cell and tissue research |