6533b872fe1ef96bd12d4306
RESEARCH PRODUCT
Contribution of polyadenylate sequences to the translational efficiency of globin messenger RNAs
A. Parets SolerRafael SentandreuDaniel GozalboJesús Zuecosubject
Translational efficiencyMolecular Sequence DataBiologyBiochemistryChromatography AffinityReticulocytePolysomeProtein biosynthesismedicinePolyadenylateNucleotideRNA MessengerGlobinMolecular Biologychemistry.chemical_classificationMessenger RNABase SequenceDNACell BiologyMolecular biologyGlobinsmedicine.anatomical_structureBiochemistrychemistryProtein BiosynthesisPotassiumElectrophoresis Polyacrylamide GelPoly AResearch Articledescription
mRNAs from reticulocyte polysomes were fractionated by chromatography on poly(U)-Sepharose and thermal elution. The molar ratio of alpha- to beta-globin mRNA was found to be 2:1 and 1:1 respectively in short- and long-poly(A) size classes. Translational analyses indicated that the globin mRNAs containing long poly(A) tracts (with a mean length of about 70 nucleotides) directed protein synthesis with higher rates than did mRNA containing short poly(A) tracts (15-35 nucleotides). Experiments performed with sub-saturating mRNA concentrations showed that the digestion with RNAase H induced a decrease in the translational capacity of both globin mRNAs and an increase in the alpha- to beta-globin synthesis ratio. No correlation was observed between the size of the poly(A) tail in mRNA and the optimal K+ requirement for translation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1987-09-15 | Biochemical Journal |