6533b872fe1ef96bd12d435b
RESEARCH PRODUCT
Glutathione metabolism in primary astrocyte cultures: flow cytometric evidence of heterogeneous distribution of GSH content.
Concha GarcíaJuan R. Vin˜aAmparo DevesaImmaculada R. PuertesJosé-enrique O'connorsubject
AntimetabolitesNerve Tissue ProteinsBiologyFlow cytometrychemistry.chemical_compoundCytosolMethionine SulfoximinemedicineAnimalsButhionine sulfoximineRats WistarMolecular BiologyButhionine SulfoximineCells CulturedBrain ChemistryCerebral Cortexmedicine.diagnostic_testGeneral NeuroscienceCell CycleGlutathioneMetabolismDNAHydrogen-Ion ConcentrationFlow CytometryGlutathioneRatsCytosolmedicine.anatomical_structurechemistryBiochemistryAnimals NewbornAstrocytesNeurogliaNeurology (clinical)IntracellularDevelopmental BiologyAstrocyteHalf-Lifedescription
The time-course of intracellular glutathione (GSH) values after incubation with L-buthionine-(S,R)-sulfoximine (BSO), a selective inhibitor of gamma-glutamylcysteine synthetase, showed that glutathione turns over with a half-life of 5 h. Intracellular GSH was assayed by flow cytometry using three different methods. Astrocytes showed a narrow range of cellular size but a wide range of intracellular GSH. This heterogeneity was resolved into three distinct subpopulations which represent 20%, 35% and 45% of the total astrocyte number. The less abundant subpopulation had the lower GSH content, while the most abundant was the subpopulation with the higher content. Over 95% of astrocytes were in the G0/G1 phase of the cell cycle, the distribution of cytosolic pH was homogeneous and the number of viable cells at the time of the assay was 90%. These results show that several pools exist when astrocyte GSH is considered and these findings may be relevant to the understanding of brain GSH metabolism.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-08-06 | Brain research |