6533b873fe1ef96bd12d4b19

RESEARCH PRODUCT

Capillary zone electrophoresis of alkaloids

Matthias Unger

subject

Indole testElectrosprayAnalyteChromatographyHydrogen bondDimerOrganic ChemistryGeneral MedicineMass spectrometryBiochemistryAnalytical Chemistrychemistry.chemical_compoundElectrophoresisCapillary electrophoresischemistry

description

Abstract A comprehensive discussion of important aspects for the analysis of alkaloids by capillary zone electrophoresis (CZE) is given. The influence of structure on the electrophoretic mobility (EM) of indole alkaloids was investigated using a running buffer which is generally applicable to the CZE analysis of alkaloids. The EM, which at the applied conditions was mostly dependent on the size and shape of the solvated analyte ions, was additionally affected by the presence of hydrogen bonds or ion–dipole interactions between protonated and unprotonated alkaloids of the same species. This could be derived from the existence of alkaloidal dimer cluster ions [2M+H]+ when mass spectrometry was used for detection.

yearjournalcountryeditionlanguage
1998-05-01Journal of Chromatography A
https://doi.org/10.1016/s0021-9673(98)00066-1