6533b873fe1ef96bd12d4b1b
RESEARCH PRODUCT
Determination of catecholamines in urine by micellar liquid chromatography with coulometric detection
J. M. Sanchis MallolsR. M. Villanueva CamañasGuillermo Ramis-ramosJ. R. Torres Lapasiósubject
Detection limitChromatographyChemistryElutionSodiumOrganic ChemistryClinical BiochemistryExtraction (chemistry)chemistry.chemical_elementBiochemistryHigh-performance liquid chromatographyAnalytical ChemistryCoulometryMicellar liquid chromatographyQuantitative analysis (chemistry)description
The determination of catecholamines by HPLC with a sodium dodecyl-sulphate (SDS), micellar mobile phase on a C18 column and with coulometric detection was studied. The eluate was conditioned at +0.25 and +0.00 V, and the current at −0.16V was recorded. A previously developed model which describes the chromatographic behaviour of solutes in HPLC with hybrid, micellar mobile phases was used to optimize the SDS and ethanol concentrations. A mobile phase of 0.15M SDS in a phosphate buffer of pH 3.4 and without ethanol is recommended. The limits of detection were 0.4–0.7 ng ml−1. The procedure was applied to the determination of unconjugated L-dopa, norepinephrine and dopamine in urine. Direct injection of the urine samples gave high results but the unconjugated catecholamines could be determined with a single solid-phase extraction step on an alumina column.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-11-01 | Chromatographia |