6533b873fe1ef96bd12d4c79
RESEARCH PRODUCT
Mass spectral identification of the blocked N-terminal tryptic peptide of the ATPase inhibitor from beef heart mitochondria
M. PrzybylskiA. TsugitaAnne-christine Dianouxsubject
Fast atom bombardmentATPaseBiophysicsPeptideN-formyi blocking groupSaccharomyces cerevisiaeMass spectrometryBiochemistryMass SpectrometryMitochondria HeartSpecies SpecificityStructural BiologyEndopeptidasesGeneticsAnimalsTrypsinAmino Acid SequenceMolecular BiologyPeptide sequencechemistry.chemical_classificationBeef heart mitochondriabiologyChemistryTryptic peptideProteinsCell BiologyFast atom bombardmentField desorption Amino acid sequenceATPase inhibitorPeptide FragmentsMitochondriaProton-Translocating ATPasesBiochemistrybiology.proteinCattledescription
AbstractThe presence of a formyl blocking group at the N-terminus of the ATPase inhibitor has been identified and the partial sequence of the N-terminal peptide has been determined by fast atom bombardment and field desorption coupled to mass spectrometry. Minor discrepancies in amino acid sequence of the inhibitor between the present and published data [(1981) Proc. Natl. Acad. Sci. USA 78, 7403-7407] are reported and its relationships with other inhbitors are briefly discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1984-08-20 | FEBS Letters |