0000000000405347

AUTHOR

A. Tsugita

showing 2 related works from this author

Exopeptidase digestion in combination with field desorption mass spectrometry for amino acid sequence determination

1982

Numerous studies have been devoted in the last years to the development of mass spectrometric methods for the sequence determination of peptides [ 1,2]. Most advanced among this work has been so far the rigorous chemical derivatization of oligopeptides to achieve sufficient volatility for the application of conventional (electron impact, EI) mass spectrometry [2-41. For example, the analysis by gas chromatography-mass spectrometry (GC-MS) of mixtures of oligopeptide fragments derivatized after chemical or enzymatic hydrolysis of polypeptides has been successfully used for sequence determinations [2,5]. Major limitations of this approach are that only small peptide derivatives are amenable t…

Exopeptidase activityChromatographyProtein mass spectrometrybiologyChemistryBiophysicsProteinsCell BiologyExopeptidaseMass spectrometryBiochemistryMass SpectrometrySample preparation in mass spectrometryStructural BiologyField desorptionExopeptidasesGeneticsbiology.proteinMass spectrumAmino Acid SequenceMolecular BiologyPeptide sequencePeptide HydrolasesFEBS Letters
researchProduct

Mass spectral identification of the blocked N-terminal tryptic peptide of the ATPase inhibitor from beef heart mitochondria

1984

AbstractThe presence of a formyl blocking group at the N-terminus of the ATPase inhibitor has been identified and the partial sequence of the N-terminal peptide has been determined by fast atom bombardment and field desorption coupled to mass spectrometry. Minor discrepancies in amino acid sequence of the inhibitor between the present and published data [(1981) Proc. Natl. Acad. Sci. USA 78, 7403-7407] are reported and its relationships with other inhbitors are briefly discussed.

Fast atom bombardmentATPaseBiophysicsPeptideN-formyi blocking groupSaccharomyces cerevisiaeMass spectrometryBiochemistryMass SpectrometryMitochondria HeartSpecies SpecificityStructural BiologyEndopeptidasesGeneticsAnimalsTrypsinAmino Acid SequenceMolecular BiologyPeptide sequencechemistry.chemical_classificationBeef heart mitochondriabiologyChemistryTryptic peptideProteinsCell BiologyFast atom bombardmentField desorption Amino acid sequenceATPase inhibitorPeptide FragmentsMitochondriaProton-Translocating ATPasesBiochemistrybiology.proteinCattleFEBS Letters
researchProduct