Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

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RESEARCH PRODUCT

Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

F.x. Lopez-labrador J.r. Brown N. Fischer H. Harvala S. Van Boheemen O. Cinek A. Sayiner T.v. Madsen E. Auvinen V. Kufner M. Huber C. Rodriguez M. Jonges M. Honemann P. Susi H. Sousa P.e. Klapper A. Perez-cataluna M. Hernandez R. Molenkamp L. Van Der Hoek R. Schuurman N. Couto K. Leuzinger P. Simmonds M. Beer D. Hoper S. Kamminga M.c.w. Feltkamp J. Rodriguez-diaz E. Keyaerts X.c. Nielsen E. Puchhammer-stockl A.c.m. Kroes J. Buesa J. Breuer E.c.j. Claas Jutte J.c. De Vries Escv Network Next-generation Seque

subject

0301 basic medicine Pathogen detection Standardization Computer science 030106 microbiology Recommendations INFLUENZA-A VIRUS DIAGNOSIS VALIDATION DNA sequencing 03 medical and health sciences 0302 clinical medicine SDG 3 - Good Health and Well-being Virology Wet lab Viral metagenomics 030212 general & internal medicine 11832 Microbiology and virology Laboratory methods High-throughput sequencing Quality assessment Network on High-Throughput Nucleotide Sequencing DNA EFFICIENT TRANSLATION Data science 3. Good health Infectious Diseases Metagenomics Viruses Next-generation sequencing 3111 Biomedicine Metagenomics DEPLETION MESSENGER-RNA Clinical virology PATHOGEN DETECTION

description

Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.

year	journal	country	edition	language
2020-06-13	Journal of Clinical Virology

10.1016/j.jcv.2020.104691 https://doi.org/10.1016/j.jcv.2020.104691