6533b873fe1ef96bd12d4e49

RESEARCH PRODUCT

Evidence for the attachment of Hsp150/Pir2 to the cell wall of Saccharomyces cerevisiae through disulfide bridges.

Ismaã­l MoukadiriJesãºs Zueco

subject

Growth mediumSaccharomyces cerevisiae ProteinsbiologySaccharomyces cerevisiaeDisulfide bondGeneral MedicineSaccharomyces cerevisiaebiology.organism_classificationApplied Microbiology and BiotechnologyMicrobiologyCell wallFungal Proteinschemistry.chemical_compoundchemistryBiochemistryCell WallDisulfides2-MercaptoethanolHeat-Shock ProteinsGlycoproteinsMercaptoethanol

description

Here we present evidence that Hsp150/Pir2, a member of the Pir family of cell wall proteins, can be extracted from the purified cell walls of Saccharomyces cerevisiae by treatment with beta-mercaptoethanol, demonstrating that at least part of this protein is attached to the cell wall through disulfide bridges. We also present evidence that Pir4, another member of this family, is partly secreted to the growth medium. Finally we propose a hypothesis to explain the relationship between the differently localized forms of particular members of the Pir family of cell wall proteins.

yearjournalcountryeditionlanguage
2001-12-01FEMS yeast research
10.1111/j.1567-1364.2001.tb00040.xhttps://pubmed.ncbi.nlm.nih.gov/12702350