0000000000860094

AUTHOR

Jesãºs Zueco

showing 2 related works from this author

O-linked mannose composition of secreted invertase of Saccharomyces cerevisiae

1989

The secreted invertase (EC 3.2.1.26) of Saccharomyces cerevisiae is a glycoenzyme that contains N- and O-linked mannoses in 40/1 proportion. The small amount of mannose chains O-linked to invertase is distributed as follows: mannose (20%), mannobiose (50%), mannotriose (6%), mannotetraose (7%) and mannopentaose (17%).

chemistry.chemical_classificationGlycosylationGlycoside Hydrolasesbeta-FructofuranosidasebiologySaccharomyces cerevisiaeMannoseSaccharomyces cerevisiaebiology.organism_classificationMicrobiologyYeastMicrobiologychemistry.chemical_compoundInvertaseEnzymechemistryBiochemistryGeneticsMannobioseComposition (visual arts)SecretionMannoseMolecular BiologyFEMS Microbiology Letters
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Evidence for the attachment of Hsp150/Pir2 to the cell wall of Saccharomyces cerevisiae through disulfide bridges.

2001

Here we present evidence that Hsp150/Pir2, a member of the Pir family of cell wall proteins, can be extracted from the purified cell walls of Saccharomyces cerevisiae by treatment with beta-mercaptoethanol, demonstrating that at least part of this protein is attached to the cell wall through disulfide bridges. We also present evidence that Pir4, another member of this family, is partly secreted to the growth medium. Finally we propose a hypothesis to explain the relationship between the differently localized forms of particular members of the Pir family of cell wall proteins.

Growth mediumSaccharomyces cerevisiae ProteinsbiologySaccharomyces cerevisiaeDisulfide bondGeneral MedicineSaccharomyces cerevisiaebiology.organism_classificationApplied Microbiology and BiotechnologyMicrobiologyCell wallFungal Proteinschemistry.chemical_compoundchemistryBiochemistryCell WallDisulfides2-MercaptoethanolHeat-Shock ProteinsGlycoproteinsMercaptoethanolFEMS yeast research
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