New adsorbents for thymidylate synthase affinity chromatography

6533b873fe1ef96bd12d4fe0

RESEARCH PRODUCT

New adsorbents for thymidylate synthase affinity chromatography

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New affinity adsorbents, intended for chromatography of thymidylate synthase (EC 2.1.1.45) from different sources, consisting of p-[N-[(2-amino-4-hydroxy-6-quinazolinyl)-methyl]-N-2-propynylamino]benzoyl-γ-[α-(3-carboxypropylamino)]glutamyl-glutamyl immobilized either on macroporous copolymer of acrylonitrile and n-butyl acrylate or on macroporous polymer of acrylonitrile itself, both crosslinked with divinylbenzene and having aminoethyl groups, were obtained. Both adsorbents were found to be effective in dUMP-dependent binding of thymidylate synthase from regenerating rat liver.

year	journal	country	edition	language
1988-01-01	Collection of Czechoslovak Chemical Communications

http://cccc.uochb.cas.cz/53/11/2890/

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