Label-free 1-DE-LC-MS/MS to identify arbuscular mycorrhiza-related membrane proteins

6533b873fe1ef96bd12d53b6

RESEARCH PRODUCT

Label-free 1-DE-LC-MS/MS to identify arbuscular mycorrhiza-related membrane proteins

Cosette Abdallah Jenny Renaut Benoît Valot Michel Zivy Ghislaine Recorbet Daniel Wipf Eliane Dumas-gaudot

subject

[SDV] Life Sciences [q-bio][SDE] Environmental Sciences root membrane proteome spectral counting [SDV]Life Sciences [q-bio]fungi [SDE]Environmental Sciences [SDV.BV]Life Sciences [q-bio]/Vegetal Biology [SDV.BV] Life Sciences [q-bio]/Vegetal Biology medicago truncatula rhizophagus irregularis

description

Deep changes in the shape and number of organelles, together with profound modifications in various membrane compartments, are induced within arbuscular mycorrhizal (AM) symbiosis. In this context, to investigate the membrane-associated proteins that are regulated in the model interaction Medicago truncatula – Rhizophagus irregularis, label-free 1DE-LC-MS/MS approach has been employed as alternative to two-dimensional gel electrophoresis. The existence of a correlation between protein abundance and peak areas or number of MS/MS spectra has widened the choice of label-free quantitative proteomics. The results highlighted microsomal protein candidates that could be involved in the symbiotic exchanges between plant and fungal cells.

year	journal	country	edition	language
2012-09-26

https://hal.inrae.fr/hal-02747762

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