6533b873fe1ef96bd12d5555
RESEARCH PRODUCT
A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting
Giovanna PitarresiFabio Salvatore PalumboAntonella Bavuso VolpeGaetano GiammonaFlavia Bongiovìsubject
Polymers and PlasticsAtom-transfer radical-polymerizationBioengineeringMethacrylateBiomaterialschemistry.chemical_compoundMonomerchemistryDynamic light scatteringPolymerizationHyaluronic acidPolymer chemistryMaterials ChemistryZeta potentialSurface chargeBiotechnologydescription
Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA-EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA-EDA-pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-07-01 | Macromolecular Bioscience |