6533b873fe1ef96bd12d563d

RESEARCH PRODUCT

Functional analysis of 12 ORFs fromSaccharomyces cerevisiae chromosome II

J. E. Perez-ortinJ. E. Perez-ortinFrancisco EstruchFrancisco EstruchSusana Rodríguez-navarroSusana Rodríguez-navarro

subject

GeneticsFunctional analysisbiologySaccharomyces cerevisiaeChromosomeBioengineeringbiology.organism_classificationApplied Microbiology and BiotechnologyBiochemistryGeneticsORFSPloidyTetradGeneFunctional genomicsBiotechnology

description

Twelve different ORFs have been deleted from the right arm of Saccharomyces cerevisiae chromosome II; namely YBR193c, YBR194w, YBR197c, YBR198c, YBR201w, YBR203w, YBR207w, YBR209w, YBR210w, YBR211c, YBR217w and YBR228w. Tetrad analysis of heterozygous deletant strains revealed that YBR193c, YBR198c and YBR211c are essential genes for vegetative growth. No effects were detected in any of the haploid deletion mutants for the rest of the ORFs with respect to growth, gross morphology or mating.

https://doi.org/10.1002/(sici)1097-0061(199907)15:10b<913::aid-yea386>3.0.co;2-a