6533b873fe1ef96bd12d58a5
RESEARCH PRODUCT
The time course of recombinant production in Streptomyces coelicolor.
G. SermontiGiuseppina FicarraA. Maria Pugliasubject
MaleTime FactorsHyphaBiologyGenetic recombinationlaw.inventionlawGeneticsMyceliumCrosses GeneticInfertility MaleRecombination GeneticSpores BacterialFertility factor (bacteria)fungiStreptomyces coelicolorChromosomeChromosome MappingGeneral MedicineChromosomes Bacterialbiology.organism_classificationMolecular biologyStreptomycesSporePhenotypeConjugation GeneticMutationRecombinant DNAdescription
SUMMARYThe process leading to gene recombination can be interrupted in the filamentous bacteriaStreptomyces coelicolorby growing mixed cultures on cellophane disks lying on complete medium. The mycelium is harvested, broken, diluted and the broken hyphae plated at different time intervals. By this means some markers can be excluded from heteroclones or from recombinant progeny in early samples. The recombinant pattern clearly changes with time, with an increase of markers contributed to the recombinant progeny. In crosses between male (NF) and female (UF) strains, the maleness is the first donor trait to appear in the cells of the recipient parent. The fertility factor does not produce a transfer origin on the donor chromosomes; the donor contribution may extend on either side or on both sides of the factor which appears to be compulsory for zygote formation. The longer the time of contact between parental cells, the longer the segment of the donor chromosome contributing to the recombinant progeny. When spores are formed they contain almost exclusively recombinant nuclei derived from segregation processes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1971-10-01 | Genetical research |