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RESEARCH PRODUCT
Cadmium induces the expression of specific stress proteins in sea urchin embryos.
Maria AgnelloValeria MatrangaRosa BonaventuraMaria Carmela Roccherisubject
Embryo NonmammalianBiophysicschemistry.chemical_elementWestern blotBiologyEmbryo developmentBiochemistryGel electrophoresiParacentrotus lividusStress proteins; Embryo development; Gel electrophoresis; Western blotWestern blotCadmium ChloridemedicineMorphogenesisStress ProteinsAnimalsElectrophoresis Gel Two-DimensionalSettore BIO/06 - Anatomia Comparata E CitologiaMolecular BiologyCells CulturedHeat-Shock ProteinsGel electrophoresisCadmiummedicine.diagnostic_testStress proteinEmbryogenesisCell BiologyGastrulaSea urchin embryoBlastulabiology.organism_classificationMolecular biologyCell biologyHighly sensitiveKineticschemistryFertilizationSea UrchinsFemaledescription
Abstract Marine organisms are highly sensitive to many environmental stresses, and consequently, the analysis of their bio-molecular responses to different stress agents is very important for the understanding of putative repair mechanisms. Sea urchin embryos represent a simple though significant model system to test how specific stress can simultaneously affect development and protein expression. Here, we used Paracentrotus lividus sea urchin embryos to study the effects of time-dependent continuous exposure to subacute/sublethal cadmium concentrations. We found that, between 15 and 24 h of exposure, the synthesis of a specific set of stress proteins (90, 72–70, 56, 28, and 25 kDa) was induced, with an increase in the rate of synthesis of 72–70 kDa (hsps), 56 kDa (hsp), and 25 kDa, which was dependent on the lengths of treatment. Recovery experiments in which cadmium was removed showed that while stress proteins continued to be synthesized, embryo development was resumed only after short lengths of exposure.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-01-01 | Biochemical and biophysical research communications |