6533b874fe1ef96bd12d6364
RESEARCH PRODUCT
Colorimetric susceptibility testing for Aspergillus fumigatus: comparison of menadione-augmented 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide and Alamar blue tests
A StübenSucharit BhakdiBernhard Jahnsubject
Microbiology (medical)Antifungal AgentsTetrazolium SaltsOxazinesMicrobial Sensitivity TestsAspergillus fumigatuschemistry.chemical_compoundMenadioneBromideAmphotericin BAmphotericin BCandida albicansOxazinesmedicineColoring AgentsCandida albicansColorimetrychemistry.chemical_classificationChromatographyDose-Response Relationship DrugbiologyAspergillus fumigatusbiology.organism_classificationIn vitroThiazolesXanthenesBiochemistrychemistryResearch Articlemedicine.drugdescription
Two colorimetric methods that use Alamar Blue or 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) for assaying the in vitro activities of antifungal agents have been described. We report that both tests performed similarly when the antifungal activity of amphotericin B against Candida albicans was determined. However, only the MTT test generated interpretable data when Aspergillus fumigatus was used.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-08-01 | Journal of Clinical Microbiology |