6533b881fe1ef96bd12d92ef

RESEARCH PRODUCT

Additional file 7 of Cellular response to spinal cord injury in regenerative and non-regenerative stages in Xenopus laevis

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Additional file 7: Figure S7. Transgenic line Xla.Tg(Dre.gfap:mCherry-Nitroreductase) allows selective cell ablation. (A) Diagram of injection and electroporation of the spinal cord at NF stage 50, indicating volume, concentration and parameters of electroporation. (B) Scheme of electroporation of the Dre.gfap:mCherry-Nitroreductase construct and treatment with vehicle or metronidazol (MTZ) at NF stage 50. (C-R) mCherry (red) expression in the spinal cord of animal electroporated at (C-D; I-J) 2 days post electroporation (dpe), before treatment; (E-F; K-L) 4 dpe and 2 days post treatment (dtt); (G-H; M-N) 7 dpe and 5 dtt, and (O- R) at 8 dpe and 6 dtt co-stained with Hoechst (blue). (S) The construct used to generate the transgenic line Xla.Tg(Dre.gfap:mCherry-Nitroreductase). (T, U) mCherry expression in the eye (arrow) and the brain of the transgenic animal at NF stage 42.