6533b883fe1ef96bd12dd616
RESEARCH PRODUCT
Morphological determination of the phototrophic community composition of biological soil crusts in coastal sand dunes in northern Germany
Sandra KammannUlf KarstenKarin GlaserUlf SchiefelbeinChristiane HassenrückTatiana MikhailyukEduardo DemchenkoChristian DolnikPeter Leinwebersubject
Nostoc cf linckiaNodosilinea cf. epilithicaTetracystis cf. sarcinalisCylindrocystis cf crassaPtychostomum moravicumElliptochloris subsphaericaThin layer chromatographyAsterococcus spCylindrocystis cf. crassaCladonia phyllophoraChloroidium cf ellipsoideumChlorolobion sp.Hypnum cupressiforme var lacunosumHypogymnia physodesStichococcus cf. bacillarisEremochloris spCrustsParmelia sulcataStenomitos sp.algaeBracteacoccus sp.Chlorococcum spPtychostomum capillarewith Nomarski DIC opticsCladonia chlorophaeaCladonia foliaceaBiospheric SciencesMyrmecia cf irregularisField experimentNodosilinea cf epilithicaBacidina etayanaPolytrichum juniperinumCoccomyxa sp.Placynthiella uliginosaLeptolyngbya spPohlia nutansCladonia furcataPeltigera extenuataWatanabea cf. acidophilaGeosciencesChloroidium cf. ellipsoideumLongitude of eventWatanabea cf acidophilaParietochloris cf. alveolarisPtychostomum imbricatulumSiteLight microscope Olympus Ltd BX53 with Nomarski DIC opticsDicranum scopariumSyntrichia ruraliformisBX53Pseudochlorella spKlebsormidium cf flaccidumOlympus LtdCladonia novochlorophaeaDATE TIMECladonia cocciferaCladonia conistaNostoc cf. linckiaDiplosphaera chodatii16S rRNAOxyrrhynchium praelongumTetracystis cf sarcinalisChlorokybus atmophyticusNostoc sp.Interfilum cf. massjukiaeMicarea misellaCoelastrella spStenomitos spNostoc spEvent labelLecanora hageniiPolytrichum piliferumTetradesmus arenicolaElevation of eventPlanophila spChlorococcum sp.Campylopus introflexusCladonia ramulosaCephaloziella divaricataPseudochlorella sp.Nostoc cf. communeChlorolobion spNannochloris spCladonia rangiformisMacrochloris spNannochloris sp.Cladonia fimbriataEvernia prunastriCaloplaca cerinellaLecania cyrtellaLecanora persimilisCladonia subulataPseudoscleropodium purumStichococcus cf bacillarisLatitude of eventLight microscopeInterfilum terricolaCladonia glaucaCladonia portentosaPlanophila sp.Spongiochloris spCladonia reisediment analysisCladonia gracilisHypnum cupressiformeTolypothrix cf. byssoideaCeratodon purpureusEcologyCladonia uncialis ssp biuncialisPseudomuriella cf. aurantiacaCylindrocystis sp.Klebsormidium cf. subtileRacomitrium canescensMicrocoleus vaginatusCoccomyxa spCylindrocystis spLobochlamys sp.soil ecologyXanthoria parietinaCladonia ciliataHypnum cupressiforme var. lacunosumActinochloris terrestrisInterfilum cf massjukiaeNatural SciencesChlorella vulgarisMacrochloris sp.Coelastrella sp.Bracteacoccus spRacomitrium elongatumCladonia scabriusculaNostoc cf. edaphicumStichococcus allasLocationNostoc cf edaphicumKlebsormidium cf. flaccidumLophozia bicuspidataPseudomuriella cf aurantiacaKlebsormidium crenulatumduneNostoc cf communeLeptolyngbya sp.Ptilidium ciliatumCladonia macilentaMyrmecia cf. irregularisPtychostomum compactumLobochlamys spPleurozium schreberiCladonia arbusculaPhyscia tenellaBrachythecium albicansPeltigera hymeniaAmandinea punctataTimaviella sp.Scoliciosporum galluraeParietochloris cf alveolarisAsterococcus sp.Hennediella heimiiTimaviella spDATE/TIMECladonia humilisBryum capillareTolypothrix cf byssoideaKlebsormidium cf subtileEremochloris sp.Cladonia floerkeanaSpongiochloris sp.Cladonia uncialis ssp. biuncialisHypnum jutlandicumdescription
This dataset comprises the microbial community composition of biological soil crusts in north-German sand dunes. For this we obtained enrichment cultures of phototrophic microorganisms, by placing fragments of biocrusts of the same Petri dishes as used for sequencing, in Petri dishes with Bold Basal (1N BBM) agarized medium (Bischoff and Bold 1963). Cultures were grown under standard laboratory conditions: with a 12-hour alteration of light and dark phases and irradiation of 25 μmol photons m-2 s-1 at a temperature 20 ± 5 ºС. Microscopic study of these raw cultures began in the third week of cultivation. Morphological examinations were performed using Olympus BX53 light microscope with Nomarski DIC optics (Olympus Ltd, Hamburg, Germany). Micrographs were taken with a digital camera (Olympus LC30) attached to the microscope, and processed by the Olympus software cellSens Entry. Direct microscopy of rewetted samples was performed in parallel with cultivation for evaluation of dominating species of algae and cyanobacteria in the original samples. Morphological identification of the biocrust organisms was based mainly on Ettl and Gärtner (2014) for green microalgae, and on Komárek (2013) for cyanobacteria, as well as on some monographs and papers devoted to taxonomic revisions of the taxa of interest (Darienko and Pröschold 2019). Moss and lichens samples were air-dried after collection. For determination, a microscope with a maximum magnification of 400x was used. Morphological identification of mosses followed Frahm and Frey (2004) with taxonomical reference to (Hodgetts et al. 2020). Lichens were determined according to Wirth et al. (2013). Morphologically critical species of the genus Cladonia where additionally analyzed by thin-layer chromatography according to (Culberson and Ammann 1979) in solvent system A.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2022-01-01 |