Search results for " ANA"
showing 10 items of 30612 documents
CVB3 VP1 interacts with MAT1 to inhibit cell proliferation by interfering with Cdk-activating kinase complex activity in CVB3-induced acute pancreati…
2021
Coxsackievirus B3 (CVB3) belongs to the genus Enterovirus of the family Picornaviridae and can cause acute acinar pancreatitis in adults. However, the molecular mechanisms of pathogenesis underlying CVB3-induced acute pancreatitis have remained unclear. In this study, we discovered that CVB3 capsid protein VP1 inhibited pancreatic cell proliferation and exerted strong cytopathic effects on HPAC cells. Through yeast two-hybrid, co-immunoprecipitation, and confocal microscopy, we show that Menage a trois 1 (MAT1), a subunit of the Cdk-Activating Kinase (CAK) complex involved in cell proliferation and transcription, is a novel interaction protein with CVB3 VP1. Moreover, CVB3 VP1 inhibited MAT…
Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane
2021
Herpes simplex virus capsids are assembled and packaged in the nucleus and move by diffusion through the nucleoplasm to the nuclear envelope for egress. Analyzing their motion provides conclusions not only on capsid transport but also on the properties of the nuclear environment during infection. We utilized live-cell imaging and single-particle tracking to characterize capsid motion relative to the host chromatin. The data indicate that as the chromatin was marginalized toward the nuclear envelope it presented a restrictive barrier to the capsids. However, later in infection this barrier became more permissive and the probability of capsids to enter the chromatin increased. Thus, although …
Biochemical and structural analysis of the NS5B RNA-dependent RNA polymerase of the hepatitis C virus.
2000
Hepatitis C virus (HCV), the major causative agent of chronic and sporadic non-A, non-B hepatitis worldwide, is a distinct member of the Flaviviridae virus family. These viruses have in common a plus-strand RNA genome that is replicated in the cytoplasm of the infected cell via minus-strand RNA intermediates. Owing to the lack of reliable cell culture systems and convenient animal models for HCV, the mechanisms governing RNA replication are not known. As a first step towards the development of appropriate in vitro systems, we expressed the NS5B RNA-dependent RNA polymerase (RdRp) in insect cells, purified the protein to near homogeneity and studied its biochemical properties. It is a primer…
Molecular Characterization of a Variant of Bacillus anthracis-Specific Phage AP50 with Improved Bacteriolytic Activity▿ †
2008
ABSTRACT The genome sequence of a Bacillus anthracis -specific clear plaque mutant phage, AP50c, contains 31 open reading frames spanning 14,398 bp, has two mutations compared to wild-type AP50t, and has a colinear genome architecture highly similar to that of gram-positive Tectiviridae phages. Spontaneous AP50c-resistant B. anthracis mutants exhibit a mucoid colony phenotype.
A Perturbed Cauchy Viscoelastic Problem in an Exterior Domain
2023
A Cauchy viscoelastic problem perturbed by an inverse-square potential, and posed in an exterior domain of RN, is considered under a Dirichlet boundary condition. Using nonlinear capacity estimates specifically adapted to the non-local nature of the problem, the potential function and the boundary condition, we establish sufficient conditions for the nonexistence of weak solutions.
Game-Theoretic Approach to Hölder Regularity for PDEs Involving Eigenvalues of the Hessian
2021
AbstractWe prove a local Hölder estimate for any exponent $0<\delta <\frac {1}{2}$ 0 < δ < 1 2 for solutions of the dynamic programming principle $$ \begin{array}{@{}rcl@{}} u^{\varepsilon} (x) = \sum\limits_{j=1}^{n} \alpha_{j} \underset{\dim(S)=j}{\inf} \underset{|v|=1}{\underset{v\in S}{\sup}} \frac{u^{\varepsilon} (x + \varepsilon v) + u^{\varepsilon} (x - \varepsilon v)}{2} \end{array} $$ u ε ( x ) = ∑ j = 1 n α j inf dim ( S ) = j sup v ∈ S | v | = 1 u ε ( x + ε v ) + u ε ( x − ε v ) 2 with α1,αn > 0 and α2,⋯ ,αn− 1 ≥ 0. The proof is based on a new coupling idea from game theory. As an application, we get the same regularity estimate for viscosity solutions of the PDE $…
Equivalence of viscosity and weak solutions for a $p$-parabolic equation
2019
AbstractWe study the relationship of viscosity and weak solutions to the equation $$\begin{aligned} \smash {\partial _{t}u-\varDelta _{p}u=f(Du)}, \end{aligned}$$ ∂ t u - Δ p u = f ( D u ) , where $$p>1$$ p > 1 and $$f\in C({\mathbb {R}}^{N})$$ f ∈ C ( R N ) satisfies suitable assumptions. Our main result is that bounded viscosity supersolutions coincide with bounded lower semicontinuous weak supersolutions. Moreover, we prove the lower semicontinuity of weak supersolutions when $$p\ge 2$$ p ≥ 2 .
Hölder regularity for the gradient of the inhomogeneous parabolic normalized p-Laplacian
2018
In this paper, we study an evolution equation involving the normalized [Formula: see text]-Laplacian and a bounded continuous source term. The normalized [Formula: see text]-Laplacian is in non-divergence form and arises for example from stochastic tug-of-war games with noise. We prove local [Formula: see text] regularity for the spatial gradient of the viscosity solutions. The proof is based on an improvement of flatness and proceeds by iteration.
Regularity for nonlinear stochastic games
2015
We establish regularity for functions satisfying a dynamic programming equation, which may arise for example from stochastic games or discretization schemes. Our results can also be utilized in obtaining regularity and existence results for the corresponding partial differential equations. peerReviewed
Remarks on regularity for p-Laplacian type equations in non-divergence form
2018
We study a singular or degenerate equation in non-divergence form modeled by the $p$-Laplacian, $$-|Du|^\gamma\left(\Delta u+(p-2)\Delta_\infty^N u\right)=f\ \ \ \ \text{in}\ \ \ \Omega.$$ We investigate local $C^{1,\alpha}$ regularity of viscosity solutions in the full range $\gamma>-1$ and $p>1$, and provide local $W^{2,2}$ estimates in the restricted cases where $p$ is close to 2 and $\gamma$ is close to 0.