Search results for " AR."
showing 10 items of 40354 documents
SARS-CoV-2 envelope protein topology in eukaryotic membranes
2020
Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Nt lum /Ct cyt ). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and ho…
Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme
1997
During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La protein was able to melt dsRNA substrates with either two 3'-overhangs or a single 3'- and a 5'-overhang. Double-stranded RNAs with two 5'-overhangs were not unwound, indicating that at least one 3'-overhang is required for unwinding. Sequence elements of the La protein that might be involved in dsRNA unwinding, such as an evolutionarily conserved putative ATP-binding motif and an element that is hom…
Clathrin- and Caveolin-Independent Entry of Human Papillomavirus Type 16—Involvement of Tetraspanin-Enriched Microdomains (TEMs)
2008
BACKGROUND: Infectious entry of human papillomaviruses into their host cells is an important step in the viral life cycle. For cell binding these viruses use proteoglycans as initial attachment sites. Subsequent transfer to a secondary receptor molecule seems to be involved in virus uptake. Depending on the papillomavirus subtype, it has been reported that entry occurs by clathrin- or caveolin-mediated mechanisms. Regarding human papillomavirus type 16 (HPV16), the primary etiologic agent for development of cervical cancer, clathrin-mediated endocytosis was described as infectious entry pathway. METHODOLOGY/PRINCIPAL FINDINGS: Using immunofluorescence and infection studies we show in contra…
The ALICE experiment at the CERN LHC
2008
Journal of Instrumentation 3(08), S08002 (2008). doi:10.1088/1748-0221/3/08/S08002
Conception en technologie CMOS d'un Système de Vision dédié à l'Imagerie Rapide et aux Traitements d'Images
2008
Our work presented in this thesis focuses on the design, testing and implementation of monolithics CMOS image smart sensors : The principle, performance and limitations. The hardware implementation of a vision smart system is the central link. HISIC is High Speed Image Capture with processing at pixel level. An experimental platform for instrumentation and evaluation of retina operators was conducted during this thesis. After a state of the smart sensors and CMOS retinas, the second part is dedicated to the study and design of the pixel image sensor HISIC. Two circuits were realized in CMOS technology. The first identied a new type of photo-detector, and the second, to create a prototype em…
(A,B) In vivo GCaMP6f signals recorded in layers M1, M5 and M9/10 of Mi1 (A) and Tm3 (B) neurons, before (blue, green) and after (gray, red) applicat…
2019
Sensory systems sequentially extract increasingly complex features. ON and OFF pathways, for example, encode increases or decreases of a stimulus from a common input. This ON/OFF pathway split is thought to occur at individual synaptic connections through a sign-inverting synapse in one of the pathways. Here, we show that ON selectivity is a multisynaptic process in the Drosophila visual system. A pharmacogenetics approach demonstrates that both glutamatergic inhibition through GluClα and GABAergic inhibition through Rdl mediate ON responses. Although neurons postsynaptic to the glutamatergic ON pathway input L1 lose all responses in GluClα mutants, they are resistant to a cell-type-specifi…
Fenomenologia e Cognitive Vision
2012
Vernon (2006) suggerisce di individuare un consenso in Cognitive Vision nella ricerca di capacità e attributi non-funzionali per un sistema robusto e adattivo. Questo contributo presenta una teoria informale di capacità percettive e motorie corrispondenti alle proprietà spaziali fenomeniche degli oggetti, vale a dire ai percetti la cui struttura è studiata in fenomenologia della percezione. Infine, se ne illustrano alcune implicazioni rispetto alle teorie cognitiviste e sensomotorie.
Uno stacco verso il cielo nei territori dell’EUR
2018
Il saggio, a partire dal progetto della torre Eurosky dello Studio Purini/Thermes, individua le relazioni urbane e indaga le qualità tipo-morfologiche dell'edificio realizzato come elemento di forte identità in un più ampio contesto che – messo a punto in un masterplan – definisce l’intera area di progetto dell’Europarco Castellaccio di Roma. Il progetto è una struttura metodologicamente fondata su scelte e considerazioni di carattere relazionale, geometrico e funzionale, che evidenziano punti di forza, di debolezza e di tolleranza capaci di modificare, anche nell’ambito coerente di riferimento di un possibile scenario di riconversione, l’intera area a ridosso dell’EUR.
Imagen de la tecnología transmitida por los textos de educación tecnológica
2006
Resumen tomado de la publicación Expone la transmisión por los textos de tecnología de una visión simplista y socialmente neutra de la tecnología como ciencia aplicada, ignorando o tratando muy superficialmente las complejas relaciones ciencia tecnología sociedad ambiente (CTSA). Valencia Universidad de León. Facultad de Educación. Servicio de Biblioteca; Campus de Vegazana, s. n.; 24071 León; Tel. +34987291146; Fax +34987291145; bufed@unileon.es; bufed1@unileon.es ESP
Análisis crítico de la representación del tema de la termodinámica en libros de texto de Bachillerato y Universidad
2006
Este trabajo estudia las visiones distorsionadas de la ciencia y la actividad científica que podemos encontrar en libros de Química cuando se introduce el capítulo de Termoquímica.