Search results for " ARB"
showing 10 items of 1262 documents
Gametic embryogenesis through isolated microspore culture in Corylus avellana L.
2015
Haploid technology is a valuable plant breeding tool for obtaining homozygosity particularly in woody plants. Hazelnut, the world’s sixth ranking nut tree crop is a monoecious, anemophilous species. It is characterized by a sporophytic incompatibility system that prevents production of homozygous plants with conventional methods, involving several self-pollination cycles. In this study, gametic embryogenesis, in particular isolated microspore culture, was tried with five genotypes. Two culture media were tested and four temperature stress treatments were applied to the isolated microspores that were cultivated at the vacuolated developmental stage. To our knowledge, this is the first report…
Transcriptomic responses to biotic stresses in Malus x domestica: a meta-analysis study.
2017
AbstractRNA-Seq analysis is a strong tool to gain insight into the molecular responses to biotic stresses in plants. The objective of this work is to identify specific and common molecular responses between different transcriptomic data related to fungi, virus and bacteria attacks in Malus x domestica. We analyzed seven transcriptomic datasets in Malus x domestica divided in responses to fungal pathogens, virus (Apple Stem Grooving Virus) and bacteria (Erwinia amylovora). Data were dissected using an integrated approach of pathway- and gene- set enrichment analysis, Mapman visualization tool, gene ontology analysis and inferred protein-protein interaction network. Our meta-analysis revealed…
Proposal of a Citrus translational genomic approach for early and infield detection of Flavescence dorée in Vitis
2014
Flavescence dore´e (FD) is one of the most widely known grapevine yellows disease and one of the most unabated worldwide in the viticulture sector. In this paper, we outline a strategy for developing an integrated system of technologies to enable rapid, early disease FD detection and diagnosis. We propose the deployment of a newly developed sensor device, the differential mobility spectrometer (DMS), which has shown positive results with a similar vector-borne disease in Citrus. We have previously demonstrated that the gas chromatograph DMS (GC/DMS) can distinguish various citrus diseases, and the system may also allow detection of volatile organic compound (VOC) signals from a tree of othe…
Identification of conserved genes linked to responses to abiotic stresses in leaves among different plant species
2020
As a consequence of global climate change, certain stress factors that have a negative impact on crop productivity such as heat, cold, drought and salinity are becoming increasingly prevalent. We conducted a meta-analysis to identify genes conserved across plant species involved in (1) general abiotic stress conditions, and (2) specific and unique abiotic stress factors (drought, salinity, extreme temperature) in leaf tissues. We collected raw data and re-analysed eight RNA-Seq studies using our previously published bioinformatic pipeline. A total of 68 samples were analysed. Gene set enrichment analysis was performed using MapMan and PageMan whereas DAVID (Database for Annotation, Visuali…
In Vitro Regeneration of Capparis spinosa L. by Using a Temporary Immersion System
2019
Three caper (Capparis spinosa L.) biotypes grown on the Sicilian island of Salina (38°
RNA-Seq analysis to investigate alternate bearing mechanism in Pistacia vera L
2018
Pistachio (Pistacia vera L.) production suffers a high level of alternate bearing. The mechanism underlying this negative phenomenon is different from other species, such as apple and olive. Pistachio produces a high number of inflorescence buds every year that in heavy cropping trees (“ON”) mostly fall during the kernel development phase, which occurs in July-August. Primary metabolites (i.e., carbohydrates) play a key role in the signaling related to inflorescence bud abscission. In this work, RNA-Seq was used as a tool to investigate transcriptome of inflorescence buds and fruits, sampled from branches with low (“OFF”) and high (“ON”) crop load. Reference based RNA-Seq analysis using Ara…
The priming fingerprint on the plant transcriptome investigated through meta-analysis of RNA-Seq data
2020
Plants may enter into a state of alert that allows them to deploy defensive measures in a more effective way upon stress occurrence. This phenomenon is termed defense priming, and it is started in plants with a still enigmatic priming phase in which complex molecular and physiological changes occur. During the priming phase the plant transcriptome is deeply affected, but it remains largely unclear the extent of the transcriptional changes that contribute to prime the plant. In this study, we performed a meta-analysis of publicly available RNA-Seq data obtained during different priming conditions and in different plant species in order to investigate the existence of a transcriptional "primi…
Autotetraploid Emergence via Somatic Embryogenesis in Vitis vinifera Induces Marked Morphological Changes in Shoots, Mature Leaves, and Stomata
2021
Polyploidy plays an important role in plant adaptation to biotic and abiotic stresses. Alterations of the ploidy in grapevine plants regenerated via somatic embryogenesis (SE) may provide a source of genetic variability useful for the improvement of agronomic characteristics of crops. In the grapevine, the SE induction process may cause ploidy changes without alterations in DNA profile. In the present research, tetraploid plants were observed for 9.3% of ‘Frappato’ grapevine somatic embryos regenerated in medium supplemented with the growth regulators β-naphthoxyacetic acid (10 µM) and N6-benzylaminopurine (4.4 µM). Autotetraploid plants regenerated via SE without detectable changes in the …
Modelling nonlinear dynamics of Crassulacean acid metabolism productivity and water use for global predictions
2021
Crassulacean acid metabolism (CAM) crops are important agricultural commodities in water-limited environments across the globe, yet modeling of CAM productivity lacks the sophistication of widely used C3 and C4 crop models, in part due to the complex responses of the CAM cycle to environmental conditions. This work builds on recent advances in CAM modeling to provide a framework for estimating CAM biomass yield and water use efficiency from basic principles. These advances, which integrate the CAM circadian rhythm with established models of carbon fixation, stomatal conductance, and the soil-plant-atmosphere continuum, are coupled to models of light attenuation, plant respiration, and bioma…
In Vitro Rooting of Capparis spinosa L. as Affected by Genotype and by the Proliferation Method Adopted During the Multiplication Phase
2020
The in vitro rooting of three caper (Capparis spinosa L.) selected biotypes, grown in a commercial orchard on the Sicilian island of Salina (38°