Search results for " Assembly"
showing 10 items of 289 documents
Raman Spectroscopic Signatures of Echovirus 1 Uncoating
2014
ABSTRACT In recent decades, Raman spectroscopy has entered the biological and medical fields. It enables nondestructive analysis of structural details at the molecular level and has been used to study viruses and their constituents. Here, we used Raman spectroscopy to study echovirus 1 (EV1), a small, nonenveloped human pathogen, in two different uncoating states induced by heat treatments. Raman signals of capsid proteins and RNA genome were observed from the intact virus, the uncoating intermediate, and disrupted virions. Transmission electron microscopy data revealed general structural changes between the studied particles. Compared to spectral characteristics of proteins in the intact v…
Folding induced supramolecular assembly into pH-responsive nanorods with a protein repellent shell
2018
We report the synthesis of ABA' triblock peptide-polysarcosine-peptide conjugates featuring two complementary phenylalanine-histidine pentapeptide strands A/A'. These sequences encode for antiparallel beta-sheet formation into folded conjugates, which promote the self-assembly into polysarcosine-shielded core-shell nanorods. These do not cause aggregation of serum proteins in human blood plasma underlining an enhanced stability.
Parallelized short read assembly of large genomes using de Bruijn graphs
2011
Abstract Background Next-generation sequencing technologies have given rise to the explosive increase in DNA sequencing throughput, and have promoted the recent development of de novo short read assemblers. However, existing assemblers require high execution times and a large amount of compute resources to assemble large genomes from quantities of short reads. Results We present PASHA, a parallelized short read assembler using de Bruijn graphs, which takes advantage of hybrid computing architectures consisting of both shared-memory multi-core CPUs and distributed-memory compute clusters to gain efficiency and scalability. Evaluation using three small-scale real paired-end datasets shows tha…
Kinetically Trapped Supramolecular Assembly of Perylene Dianhydride Derivative in Methanol: Optical Spectra, Morphology, and Mechanisms.
2016
Supramolecular self-assembly has attracted increasing attention as a breakthrough methodology in the fields of nanoscience and nanotechnology. Herein, a perylene dianhydride derivative (TP-PDA) self-assembles into well-defined nanospheres through a nucleation-growth process. The mechanisms of this process were explored by using spectral analysis, dynamic light scattering (DLS), and scanning electron microscopy (SEM). In situ DLS and in situ SEM both revealed that the size of the aggregated nanospheres increases with time until the formation of equilibrium H-aggregates. This shows that TP-PDA undergoes a kinetically trapped assembly with a rapid transformation into the thermodynamically favo…
The key role of hydrogen bonding in the nuclearity of three copper(II) complexes with hydrazone-derived ligands and nitrogen donor heterocycles
2011
International audience; Three new Cu(II) complexes of formula [Cu(L1)(pyz)(CH3OH)]ClO4 (1), [Cu(L1)(4,4′-bpy)(ClO4)]·0.5H2O (2) and [{Cu(L2)(ClO4)}2(μ-4,4′-bpy)] (3) have been synthesised by using pyrazine (pyz) and 4,4′-bipyridine (4,4′-bpy) and tridentate O,N,O-donor hydrazone ligands, L1H and L2H, obtained by the condensation of 1,1,1-trifluoro-2,4-pentanedione with salicyloylhydrazide and benzhydrazide, respectively. The ligands and their complexes have been characterized by elemental analyses, FT-IR, and UV–Vis spectroscopies. Single crystal X-ray structure analysis evidences the metal ion in a slightly deformed square pyramidal geometry in all the complexes. However complexes 1 and 2 …
Exploring the 2,2′-Diamino-5,5′-bipyrimidine Hydrogen-Bonding Motif: A Modular Approach to Alkoxy-Functionalized Hydrogen-Bonded Networks
1998
The programmed self-association of 2,2’-diamino-4,4’-dialkoxy-5,5’-bipyrimidines allows for the de novo construction of alkoxy-functionalized H-bonded ribbons and sheets as evidenced by X-ray crystallographic analysis. The data provide insight into the interplay of the different structural and interactional features of the molecular components to the generation of the supramolecular assembly. Hydrophobicity of the didodecyl side chains of 4c leads to the dominance of the H-bonding factor, resulting in the formation of a fully interconnected array. These results define the utility of the of 2,2’-diamino-4,4’-dialkoxy-5,5’-bipyrimidines as a potential scaffold for the attachment of electro- o…
"Comment on ""Non-symmetric substituted ureas locked in an (E,Z) conformation: an unusual anion binding via supramolecular assembly"" by M. Olivari, …
2014
We propose another point of view on the type of hydrogen bonded complexes that were described in this journal (M. Olivari et al., New J. Chem., 2013, 37, 663). The main difference is the molecular geometry and breakage of the intramolecular hydrogen bond during association. The current comment is to highlight mentioned aspects and to point out that in some cases the interpretation may not be straightforward due to the simultaneous effects associated with complexation.
Assembly and encapsulation of coordination tectons driven by hydrogen-bondingand space-filling
2001
[FR] Le composé ¿Fe(LI)3¿2¿Fe(H2O)6¿(ClO4)6 (2), LI = 1,10-phénanthroline-5,6-dione, a été synthétisé et caractérisé. La structure cristalline 2 est définie par un assemblage bidimensionnel non covalent, peu commun, constitué par des tectons chiraux ¿Fe(LI)3¿2+, assemblés par des cations ¿Fe(H2O)6¿2+ encapsulés dans des cages. Ces cages sont formées par 12 liaisons hydrogène établies entre les molécules d¿eau coordinées et les groupes dione appartenant à six molecules chirales ¿Fe(LI)3¿2+ ¿, ¿ alternées.
ChemInform Abstract: Exploring the 2,2′-Diamino-5,5′-bipyrimidine Hydrogen-Bonding Motif: A Modular Approach to Alkoxy-Functionalized Hydrogen-Bonded…
2010
The programmed self-association of 2,2’-diamino-4,4’-dialkoxy-5,5’-bipyrimidines allows for the de novo construction of alkoxy-functionalized H-bonded ribbons and sheets as evidenced by X-ray crystallographic analysis. The data provide insight into the interplay of the different structural and interactional features of the molecular components to the generation of the supramolecular assembly. Hydrophobicity of the didodecyl side chains of 4c leads to the dominance of the H-bonding factor, resulting in the formation of a fully interconnected array. These results define the utility of the of 2,2’-diamino-4,4’-dialkoxy-5,5’-bipyrimidines as a potential scaffold for the attachment of electro- o…
Nouvelles perspectives concernant la structure et la fonction du domaine carboxyl terminal de Hfq
2015
Accumulating evidence indicates that RNA metabolism components assemble into supramolecular cellular structures to mediate functional compartmentalization within the cytoplasmic membrane of the bacterial cell. This cellular compartmentalization could play important roles in the processes of RNA degradation and maturation. These components include Hfq, the RNA chaperone protein, which is involved in the post-transcriptional control of protein synthesis mainly by the virtue of its interactions with several small regulatory ncRNAs (sRNA). The Escherichia coli Hfq is structurally organized into two domains. An N-terminal domain that folds as strongly bent β-sheets within individual protomers to…