Search results for " BOX"
showing 10 items of 161 documents
Isolation and characterization of cold-shock domain protein genes, Oryzias latipes Y-box protein 2 ( OlaYP2 ) and Fugu rubripes Y-box protein 1 ( Fru…
2002
The Y-box protein (YP) family shares a nucleic acid binding domain, called cold-shock domain, that has been evolutionarily highly conserved from bacteria to human. The different YPs identified so far in vertebrates are thought to function as transcriptional activators, transcriptional repressors and/or translational repressors. Medakafish and pufferfish are very suitable vertebrate models for the study of developmental genetics and comparative genomics, respectively. Here we report the isolation of two teleost YP genes, medakafish Oryzias latipes (Ola)YP2 and Fugu rubripes (Fru)YP1, which are expressed in multiple tissues. Phylogenetic analysis demonstrated that OlaYP2 and FruYP1 belong to …
Two Cases of Thyroid Dysgenesis Caused by Different Novel PAX8 Mutations in the DNA-Binding Region: In Vitro Studies Reveal Different Pathogenic Mech…
2013
Mutations in PAX8, a transcription factor gene, cause thyroid dysgenesis (TD). The extreme variability of the thyroid phenotype makes it difficult to identify individuals harboring PAX8 gene mutations. Here we describe two patients with TD and report two novel PAX8 gene mutations (S54R and R133Q). We performed in vitro studies to functionally characterize these mutations.Using PAX8 expression vectors, we investigated whether the PAX8 mutants localized correctly to the nucleus. To analyze the DNA-binding properties of S54R and R133Q, electrophoretic mobility shift assays were performed. Furthermore, we measured whether the mutant PAX8 proteins were able to activate the thyroglobulin (TG)- an…
Effects of housing and nicotine on shuttle-box avoidance in male NMRI mice
2005
The present study aimed to evaluate whether housing condition could interact with nicotine administration in influencing the acquisition of a two-way active avoidance task. Male NMRI mice were either group- or individually housed for 30 days and, after this period, evaluated both in the actimeter and, 24h later, in the elevated plus-maze. On the basis of the percentage of time spent in the open arms of the plus-maze, both group- and individually housed mice were sub-classified into three groups with high, moderate or low anxiety baseline levels. Effects of nicotine on the acquisition of the two-way active avoidance task was assessed in each of these groups of mice using an automated shuttle…
The Embodied Attunement of Therapists and a Couple within Dialogical Psychotherapy: An Introduction to the Relational Mind Research Project
2015
In dialogical practice, therapists seek to respond to the utterances of clients by including in their own response what the client said. No research so far exists on how, in dialogs, therapists and clients attune themselves to each other with their entire bodies. The research program The Relational Mind is the first to look at dialog in terms of both the outer and the inner dialogs of participants (clients and therapists), observed in parallel with autonomic nervous system (ANS) measurements. In the ANS, the response occurs immediately, even before conscious thought, making it possible to follow how participants in a multiactor dialog synchronize their reactions and attune themselves to eac…
Mandibular-pelvic-patellar syndrome (mpp) is a novel pitx1-related disorder due to alteration of pitx1 transactivation ability
2020
International audience; PITX1 is a homeobox transcription factor essential for hindlimb morphogenesis. Two PITX1-related human disorders have been reported to date: PITX1 ectopic expression causes Liebenberg syndrome, characterized by malformation of upper limbs showing a "lower limb" appearance; PITX1 deletions or missense variation cause a syndromic picture including clubfoot, tibial hemimelia, and preaxial polydactyly. We report two novel PITX1 missense variants, altering PITX1 transactivation ability, in three individuals from two unrelated families showing a distinct recognizable autosomal dominant syndrome, including first branchial arch, pelvic, patellar, and male genital abnormaliti…
Altered REDD1, myostatin, and Akt/mTOR/FoxO/MAPK signaling in streptozotocin-induced diabetic muscle atrophy
2011
Type 1 diabetes, if poorly controlled, leads to skeletal muscle atrophy, decreasing the quality of life. We aimed to search highly responsive genes in diabetic muscle atrophy in a common diabetes model and to further characterize associated signaling pathways. Mice were killed 1, 3, or 5 wk after streptozotocin or control. Gene expression of calf muscles was analyzed using microarray and protein signaling with Western blotting. We identified translational repressor protein REDD1 (regulated in development and DNA damage responses) that increased seven- to eightfold and was associated with muscle atrophy in diabetes. The diabetes-induced increase in REDD1 was confirmed at the protein level. …
AMaLGaM IDEAs in noiseless black-box optimization benchmarking
2009
This paper describes the application of a Gaussian Estimation-of-Distribution (EDA) for real-valued optimization to the noiseless part of a benchmark introduced in 2009 called BBOB (Black-Box Optimization Benchmarking). Specifically, the EDA considered here is the recently introduced parameter-free version of the Adapted Maximum-Likelihood Gaussian Model Iterated Density-Estimation Evolutionary Algorithm (AMaLGaM-IDEA). Also the version with incremental model building (iAMaLGaM-IDEA) is considered.
DEA-like Models for the Efficiency Evaluation of Hierarchically Structured Units
2004
Abstract The knowledge of the internal structure of decision making units (DMUs) gives further insights with respect to the “black box” perspective when considering data envelopment analysis models. We present one-level and two-level hierarchical structures of the DMUs under evaluation. Each unit is composed of consecutive stages of parallel subunits all with constant returns to scale. In particular, the maximization of the relative efficiency of a DMU is studied. For the two-stage situation, different degrees of coordination among the subunits of the hierarchical levels are discussed. When some form of coordination has to be guaranteed, we introduce balancing constraints and we compare two…
Chronological records of metal deposition in sediments from the Strait of Sicily, central Mediterranean: assessing natural fluxes and anthropogenic a…
2010
Abstract Sediment box-cores were recovered from the Strait of Sicily along two onshore–offshore transects in water depths of 29–500 m. Samples were dated by 210 Pb and analysed for major and trace elements. Inspections of chronological profiles integrated with application of statistical algorithms to the geochemical dataset and supported by in situ hydrological observations were used to assess factors driving element distributions. Mineralogical and chemical variability of sediments offshore of the southwestern Sicily coast reflect the irregular sea floor morphology of the Adventure Bank. Anthropogenic inputs explain enrichments with respect to background values for Sb, As, Pb, and Hg, that…
Functional characterization of Tat protein from human immunodeficiency virus. Evidence that Tat links viral RNAs to nuclear matrix.
1990
The processes of transcription and posttranscription are assumed to proceed in close association with the nuclear matrix. In this study we demonstrated that Tat, the trans-activating protein from human immunodeficiency virus type 1 (HIV-1), binds both to the TAR region of the nascent HIV mRNAs and the nuclear matrix with high affinity. Both North/Western blotting experiments and nitrocellulose binding studies revealed that Tat binds with an association constant (K alpha) of approximately 1 x 10(9) M-1 to the TAR segment of HIV RNA; binding of Tat to this sequence which is present between position 32 and 82 downstream from the TATA box was also confirmed by gel retardation assays. Binding of…